Localization of IAA transporting tissue by tissue printing and autoradiography. [Coleus; maize]

Conference · Wed May 01 00:00:00 EDT 1991 · Plant Physiology, Supplement; (United States)

OSTI ID:5600833

Cha, Mee-Rye; Evans, M L; Hangarter, R P ^[1]

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1. Ohio State Univ., Columbus (United States)

Tissue printing on nitrocellulose membranes provides a useful technique for visualizing anatomical details of tissue morphology of cut ends of stem segments. Basal ends of Coleus stem and corn coleoptile segments that were transporting {sup 14}C-IAA were gently blotted onto DEAE-nitrocellulose for several minutes to allow {sup 14}C-IAA to efflux from the tissue. Because of the anion exchange properties of DEAE-nitrocellulose the {sup 14}C-IAA remains on the membrane at the point it leaves the transporting tissue. Autoradiography of the DEAE membrane allowed indirect visualization of the tissues preferentially involved in auxin transport. The authors observed that polar transport through the stem segments occurred primarily through or in association with vascular tissues. However, in Coleus stems, substantial amounts of the label appeared to move through the tissue by diffusion as well as by active transport.

• OSTI ID: 5600833
• Report Number(s): CONF-9107184--
• Journal Information: Plant Physiology, Supplement; (United States), Journal Name: Plant Physiology, Supplement; (United States) Vol. 96:1; ISSN PPYSA; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
BODY
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CEREALS
DIFFUSION
DISTRIBUTION
GRAMINEAE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LILIOPSIDA
MAGNOLIOPHYTA
MAIZE
PHYSIOLOGY
PLANT GROWTH REGULATORS
PLANT TISSUES
PLANTS
TISSUE DISTRIBUTION
TISSUES
TRACER TECHNIQUES