Identification of the cells involved in auxin transport in maize mesocotyl

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6610809

Jones, A M ^[1]

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1. Univ. of North Carolina, Chapel Hill (USA)

A study was undertaken to identify by a direct method the cells involved in auxin transport through maize mesocotyl tissue. The auxin photoaffinity labeling agent, 7-({sup 3}H), 5-azidoindole 3-acetic acid (N{sub 3}IAA), was loaded into excised stem tissue from a cut end. Polar transport of this analog was demonstrated over 4 hours by comparing uptake into tissue loaded with N{sub 3}IAA from the apical vs. the basal end. Triiodobenzoic acid, an auxin transport inhibitor, inhibited N{sub 3}IAA uptake into tissue. Tissue which had taken up the photoaffinity labeling agent was photolyzed to covalently fix the radioisotope within cells. This tissue was sectioned and subjected to in situ autoradiography. The outermost cell of epidermal tissue and certain files of cells in vascular tissue were densely labeled indicating that on a per cell basis these two cell types are most actively transporting auxin.

• OSTI ID: 6610809
• Journal Information: Plant Physiology, Supplement; (USA), Journal Name: Plant Physiology, Supplement; (USA) Vol. 89:4; ISSN PPYSA; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
AUXINS
CEREALS
GRASS
HYDROGEN COMPOUNDS
LILIOPSIDA
MAGNOLIOPHYTA
MAIZE
MEMBRANE TRANSPORT
PLANT CELLS
PLANT GROWTH REGULATORS
PLANTS
TRITIUM COMPOUNDS