Antiviral T cell competence and restriction specificity of mixed allogeneic (P1 + P2----P1) irradiation chimeras

Journal Article · Thu Jun 01 04:00:00 EDT 1989 · Cellular Immunology; (USA)

DOI:https://doi.org/10.1016/0008-8749(89)90016-6· OSTI ID:5599911

Rueedi, E S; Sykes, M; Ildstad, S T; Chester, C H; Althage, A; Hengartner, H; Sachs, D H; Zinkernagel, R M ^[1]

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1. National Cancer Institute, Bethesda, MD (USA)

Mixed irradiation bone marrow chimeras were prepared by reconstituting lethally irradiated C57BL/10 (B10) or B10.D2 mice with T cell-depleted bone marrow cells of B10 plus B10.D2 origin. These chimeras were healthy and survived well under conventional housing conditions and after experimental laboratory infections. Of a total of 17 chimeras tested, 2 died spontaneously or from the injected virus. Twelve of fifteen chimeras mounted a measurable cytotoxic T cell response to virus. Despite approximately equal percentages of B10 and B10.D2 lymphocytes in chimeras, cytotoxic T cell responses to vaccinia virus and lymphocytic choriomeningitis virus were mediated variably by either syngeneic or allogeneic donor lymphocytes; thus the H-2 type of effector T cells frequently did not correspond to the 50:50 distribution of spleen or peripheral blood lymphocytes. Cytotoxic responses were restricted exclusively to recipient H-2 type. All mixed chimeras examined were able to mount a good IgG response to vesicular stomatitis virus. These results confirm previous data suggesting that such mixed chimeras are healthy and immunocompetent and demonstrate strict recipient-determined restriction specificity of effector T cells; they also suggest that if T help is necessary for induction of virus-specific cytotoxic T cells, it does not require host-restricted interactions between helper T cells and precursor cytotoxic T cells.

• OSTI ID: 5599911
• Journal Information: Cellular Immunology; (USA), Journal Name: Cellular Immunology; (USA) Vol. 121:1; ISSN 0008-8749; ISSN CLIMB
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIGENS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CHIMERAS
CONNECTIVE TISSUE CELLS
DISEASES
GLOBULINS
HEMATOPOIETIC SYSTEM
IMMUNE REACTIONS
IMMUNOGLOBULINS
INFECTIOUS DISEASES
INFECTIVITY
IRRADIATION
LETHAL IRRADIATION
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
MICROORGANISMS
MOSAICISM
ORGANIC COMPOUNDS
ORGANS
PARASITES
PROTEINS
RADIATION CHIMERAS
RODENTS
SOMATIC CELLS
SPECIFICITY
TISSUES
TOXICITY
VERTEBRATES
VIRAL DISEASES
VIRUSES