Antiviral T cell competence and restriction specificity of mixed allogeneic (P1 + P2----P1) irradiation chimeras

Journal Article · Thu Jun 01 00:00:00 EDT 1989 · Cellular Immunology; (USA)

DOI:https://doi.org/10.1016/0008-8749(89)90016-6· OSTI ID:5599911

Rueedi, E S; Sykes, M; Ildstad, S T; Chester, C H; Althage, A; Hengartner, H; Sachs, D H; Zinkernagel, R M ^[1]

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1. National Cancer Institute, Bethesda, MD (USA)

Mixed irradiation bone marrow chimeras were prepared by reconstituting lethally irradiated C57BL/10 (B10) or B10.D2 mice with T cell-depleted bone marrow cells of B10 plus B10.D2 origin. These chimeras were healthy and survived well under conventional housing conditions and after experimental laboratory infections. Of a total of 17 chimeras tested, 2 died spontaneously or from the injected virus. Twelve of fifteen chimeras mounted a measurable cytotoxic T cell response to virus. Despite approximately equal percentages of B10 and B10.D2 lymphocytes in chimeras, cytotoxic T cell responses to vaccinia virus and lymphocytic choriomeningitis virus were mediated variably by either syngeneic or allogeneic donor lymphocytes; thus the H-2 type of effector T cells frequently did not correspond to the 50:50 distribution of spleen or peripheral blood lymphocytes. Cytotoxic responses were restricted exclusively to recipient H-2 type. All mixed chimeras examined were able to mount a good IgG response to vesicular stomatitis virus. These results confirm previous data suggesting that such mixed chimeras are healthy and immunocompetent and demonstrate strict recipient-determined restriction specificity of effector T cells; they also suggest that if T help is necessary for induction of virus-specific cytotoxic T cells, it does not require host-restricted interactions between helper T cells and precursor cytotoxic T cells.

• OSTI ID: 5599911
• Journal Information: Cellular Immunology; (USA), Vol. 121:1; ISSN 0008-8749
• Country of Publication: United States
• Language: English

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SPECIFICITY
RADIATION CHIMERAS
IMMUNE REACTIONS
VIRUSES
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ANTIGENS
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GLOBULINS
HEMATOPOIETIC SYSTEM
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560152* - Radiation Effects on Animals- Animals