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Facilitation of allogeneic bone marrow transplantation by a T cell-specific immunotoxin containing daunomycin

Journal Article · · Transplantation; (United States)
; ; ; ; ;

Daunomycin coupled via an acid-sensitive spacer to monoclonal Thy-1.2-specific antibody was used to purge T lymphocytes from a 1:1 mixture of murine C57BL/6J bone marrow and spleen cells prior to engraftment in fully allogeneic, irradiated BALB/c recipients. Treatment of bone marrow with the immunotoxin at a concentration used for purging had no effect on the viability of committed hematopoietic progenitor or multipotent stem cells. All of the recipients of purged bone marrow were at least 80% chimeric for donor peripheral blood cells and none developed graft-versus-host disease. Out of 50 chimeras, 49 were still alive more than 200 days posttransplantation. The chimeras were shown to be tolerant to donor tissue as tested by mixed lymphocyte reactivity, cell-mediated cytotoxicity, and skin grafting. The same tests revealed full immunocompetence of chimeras to third-party alloantigens. In vivo IgM and IgG antibody responses to sheep red blood cells were similar in magnitude in allogeneically and syngeneically reconstituted mice.

Research Organization:
Univ. of Alberta, Edmonton
OSTI ID:
5478899
Journal Information:
Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 44:6; ISSN TRPLA
Country of Publication:
United States
Language:
English

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Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
ANTIBODY FORMATION
ANTINEOPLASTIC DRUGS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BONE MARROW CELLS
CHIMERAS
CONNECTIVE TISSUE CELLS
DRUGS
ERYTHROCYTES
FUNCTIONS
GRAFT-HOST REACTION
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
MONOCLONAL ANTIBODIES
MOSAICISM
RADIATION CHIMERAS
RODENTS
SOMATIC CELLS
SPECIFICITY
SPLEEN CELLS
STEM CELLS
TRANSPLANTS
VERTEBRATES