Effects of amyotrophic lateral sclerosis sera on cultured cholinergic neurons

Journal Article · Tue Mar 01 04:00:00 EST 1983 · Neurology; (United States)

DOI:https://doi.org/10.1212/WNL.33.3.317· OSTI ID:5598898

Touzeau, G; Kato, A C

Dissociated monolayer cultures of chick ciliary ganglion neurons have been used to study the effects of control and ALS sera. The cultured neurons survive and extend neurites for a minimum of 2 weeks in a standard tissue culture medium that contains 10% heat-inactivated human serum. Three parameters of the neurons have been examined when cultured in control and ALS sera for 8 to 12 days: (1) neuronal survival, (2) activity of the enzyme choline acetyltransferase, and (3) synthesis of /sup 3/H-acetylcholine using /sup 3/H-choline as precursor. ALS sera cause a small decrease in these three parameters, but this difference is not significant.

• Research Organization: Department of Pharmacology, Centre Medical Universitaire, Geneva, Switzerland
• OSTI ID: 5598898
• Journal Information: Neurology; (United States), Journal Name: Neurology; (United States) Vol. 33:3; ISSN NEURA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOSYNTHESIS
BLOOD SERUM
CARBOXYLESTERASES
CELL CULTURES
CHOLINESTERASE
DISEASES
DRUGS
ENZYME ACTIVITY
ENZYMES
ESTERASES
ESTERS
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NERVE CELLS
NERVOUS SYSTEM DISEASES
NEUROREGULATORS
ORGANIC COMPOUNDS
PARASYMPATHOMIMETICS
QUATERNARY COMPOUNDS
SOMATIC CELLS
SURVIVAL TIME
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS