Characterization of a putative acetylcholine receptor in chick ciliary ganglion neurons

Thesis/Dissertation · Tue Jan 01 04:00:00 EST 1985

OSTI ID:5219891

Stollberg, J

Monoclonal antibodies to the main immunogenic region on the alpha subunit of acetylcholine receptors in muscle and electric organ recognize membrane components in chick brain and ciliary ganglia that are candidates for the neuronal receptor. The component in chick brain has been purified by immunoaffinity chromatography. It specifically binds nicotine but not alpha-bungarotoxin, and can be affinity labeled with (/sup 3/H)bromoacetylcholine. The cross-reacting component in ciliary ganglion neurons is concentrated in synaptic membrane, and can be modulated by exposure of the cells to cholinergic ligands in culture. The cross-reacting component in ciliary ganglion neurons is an integral membrane component that binds concanavalin A, and it is distinct from the alpha-bungarotoxin binding component. The acetylcholine receptor function in these neurons can be locked by affinity alkylation with bromoacetylcholine, indicating similarity in this respect to receptors from muscle and electric organ. Antisera raised against the partially purified component from chick brain also block receptor function on ciliary ganglion neurons. The subcellular distribution of the ganglion component in culture is assessed, and it is shown that approximately 2/3 of the cross-reacting components are intracellular; the majority of these seem not to be destined for insertion into the plasma membrane.

• Research Organization: California Univ., San Diego (USA)
• OSTI ID: 5219891
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
AGGLUTININS
ALKALOIDS
ALKYLATION
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
ANTIBODIES
AUTONOMIC NERVOUS SYSTEM AGENTS
AZINES
AZOLES
BIOCHEMICAL REACTION KINETICS
BIRDS
BODY
BRAIN
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
CHICKENS
CHROMATOGRAPHY
CONCANAVALIN
DATA
DRUGS
ESTERS
EXPERIMENTAL DATA
FOWL
GANGLIONS
HEMAGGLUTININS
HETEROCYCLIC COMPOUNDS
IMMUNE SERUMS
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LECTINS
LIGANDS
MEMBRANE PROTEINS
MEMBRANES
MONOCLONAL ANTIBODIES
NERVE CELLS
NERVOUS SYSTEM
NEUROREGULATORS
NICOTINE
NUMERICAL DATA
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PARASYMPATHOLYTICS
PARASYMPATHOMIMETICS
PROTEINS
PURIFICATION
PYRIDINES
PYRROLES
PYRROLIDINES
QUATERNARY COMPOUNDS
RADIORECEPTOR ASSAY
REACTION KINETICS
RECEPTORS
SEPARATION PROCESSES
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES