Interaction of nicotinic receptor affinity reagents with central nervous system. cap alpha. -bungarotoxin-binding entities
Membrane-bound ..cap alpha..-bungarotoxin-binding entities derived from rat brain are found to interact specifically with the affinity reagents maleimidobenzyltrimethylammonium (MBTA) and bromoacetylcholine (BAC), originally designed to label nicotinic acetylcholine receptors from electroplax and skeletal muscle. Following treatment of membranes with dithiothreitol, all specific toxin binding sites are irreversibly blocked by reaction with MBTA or BAC. Affinity reagent labeling of dithiothreitol-reduced membranes is prevented (toxin binding sites are not blocked) by prior alkylaction with N-ethylmaleimide, by prior oxidation with dithiobis(2-nitrobenzoic acid), or by incubation with neurotoxin. Reversibly associating cholinergic agonists and antagonists retard the rate of affinity reagent interaction with toxin receptors. The apparent rates of affinity reagent alkylation of toxin receptors, and the influences of other sulfhydryl/disulfide reagents on affinity labeling are comparable to those observed for reaction with nicotinic acetylcholine receptors in the periphery. The results provide further evidence that central nervous system ..cap alpha..-bungarotoxin receptors share a remarkable number of biochemical properties with nicotinic receptors from the periphery.
- Research Organization:
- Univ. of California, Berkeley
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 6644379
- Journal Information:
- Mol. Pharmacol.; (United States), Journal Name: Mol. Pharmacol.; (United States) Vol. 17; ISSN MOPMA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Chemical modification and reactivity of sulfhydryls and disulfides of rat brain nicotinic-like acetylcholine receptors
Effects of thiol modification and Ca/sup + +/ on agonist-specific state transitions of nicotinic acetylcholine receptor from Torpedo californica electroplax
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ALKALOIDS
ALKYLATING AGENTS
ALKYLATION
AMINES
ANIMALS
ANTIGENS
ANTIMITOTIC DRUGS
AZINES
AZOLES
BINDING ENERGY
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
DRUGS
ENERGY
HETEROCYCLIC COMPOUNDS
HISTOLOGY
KINETICS
MAMMALS
MATERIALS
MEDICINE
MEMBRANES
NERVOUS SYSTEM
NEUROLOGY
NICOTINE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PYRIDINES
PYRROLES
PYRROLIDINES
RATS
REACTION KINETICS
REAGENTS
RECEPTORS
RODENTS
SENSITIVITY
THIOLS
TOXIC MATERIALS
TOXINS
VENOMS
VERTEBRATES