Comparison of three tracers for detecting lung epithelial injury in anesthetized sheep

Journal Article · Mon May 01 04:00:00 EDT 1989 · J. Appl. Physiol.; (United States)

OSTI ID:5597383

Peterson, B T; Dickerson, K D; James, H L; Miller, E J; McLarty, J W; Holiday, D B

We compared the ability of three aerosolized tracers to discriminate among control, lung inflation with a positive end expired pressure of 10 cmH/sub 2/O, lung vascular hypertension and edema without lung injury, and lung edema with lung injury due to intravenous oleic acid. The tracers were /sup 99m/Tc-diethylenetriaminepentaacetate (/sup 99m/Tc-DTPA, mol wt 492), /sup 99m/Tc-human serum albumin (/sup 99m/Tc-ALB, mol wt 69,000), and /sup 99m/Tc-aggregated albumin (/sup 99m/Tc-AGG ALB, mol wt 383,000). /sup 99m/Tc-DTPA clearance measurements were not able to discriminate lung injury from lung inflation. The /sup 99m/Tc-AGG ALB clearance rate was unchanged by lung inflation and increased slightly with lung injury. The 99mTc-ALB clearance rate (0.06 +/- 0.02%/min) was unchanged by lung inflation (0.09 +/- 0.02%/min, P greater than 0.05) or 4 h of hypertension without injury (0.09 +/- 0.04%/min, P greater than 0.05). Deposition of /sup 99m/Tc-ALB within 15 min of the administration of the oleic acid increased the clearance rate to 0.19 +/- 0.06%/min, which correlated well with the postmortem lung water volume (r = 0.92, P less than 0.01). This did not occur when there was a 60-min delay in the deposition of /sup 99m/Tc-ALB. We conclude that /sup 99m/Tc-ALB is the best indicator for studying the effects of lung epithelial injury on protein and fluid transport into and out of the air spaces of the lungs in a minimally invasive manner.

• Research Organization: Univ. of Texas Health Center, Tyler (USA)
• OSTI ID: 5597383
• Journal Information: J. Appl. Physiol.; (United States), Journal Name: J. Appl. Physiol.; (United States) Vol. 66:5; ISSN JAPYA
• Country of Publication: United States
• Language: English

Related Subjects

550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALBUMINS
AMINO ACIDS
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD PRESSURE
BODY
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CHALCOGENIDES
CHELATING AGENTS
CLEARANCE
COMPARATIVE EVALUATIONS
DIAGNOSIS
DISEASES
DRUGS
DTPA
EDEMA
ELEMENTS
EPITHELIUM
EXCRETION
HOURS LIVING RADIOISOTOPES
HYPERTENSION
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LUNG CLEARANCE
LUNGS
NONMETALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN
OXYGEN COMPOUNDS
PARTIAL PRESSURE
PATHOLOGICAL CHANGES
PROTEINS
RADIOISOTOPES
RADIOPHARMACEUTICALS
RADIOPROTECTIVE SUBSTANCES
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
SYMPTOMS
TECHNETIUM 99
TECHNETIUM ISOTOPES
TISSUES
TRACER TECHNIQUES
VASCULAR DISEASES
YEARS LIVING RADIOISOTOPES