Turbidity as a method of preparing sperm dilutions in the echinoid sperm/egg bioassay
- National Council of the Paper Industry for Air and Stream Improvement, Anacortes, WA (United States)
The use of turbidimeter for preparing sperm dilutions used in the echinoid sperm/egg bioassay was evaluated. Regression analyses of the relationship between sperm density and turbidity for the sea urchins Strongylocentrotus purpuratus and Strongylocentrotus droebachiensis and the sand dollar Dendraster excentricus indicated that although there were slope differences for each species, each coefficient of determination was highly significant. For Dendraster excentricus, triplicate hemacytometer counts over a range of turbidities as well as repeated preparations of a single sperm turbidity indicated similar variability for each. The use of the turbidimeter has time-saving advantages over conventional hemacytometer methods without sacrificing precision. Sperm dilutions can be prepared rapidly, minimizing seawater sperm preactivation before test initiation, and may therefore contribute to increased test precision.
- OSTI ID:
- 5596827
- Journal Information:
- Environmental Toxicology and Chemistry; (United States), Vol. 12:11; ISSN 0730-7268
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHEMICAL EFFLUENTS
BIOASSAY
ECHINODERMS
SENSITIVITY
SEAWATER
TURBIDITY
EGGS
GENETIC VARIABILITY
REGRESSION ANALYSIS
SEA URCHINS
SPERMATOZOA
WATER POLLUTION
ANIMALS
AQUATIC ORGANISMS
BENTHOS
BIOLOGICAL VARIABILITY
CHEMICAL WASTES
GAMETES
GERM CELLS
HYDROGEN COMPOUNDS
INVERTEBRATES
MATHEMATICS
NONRADIOACTIVE WASTES
OXYGEN COMPOUNDS
POLLUTION
STATISTICS
WASTES
WATER
540320* - Environment
Aquatic- Chemicals Monitoring & Transport- (1990-)
560300 - Chemicals Metabolism & Toxicology