Application of toxicity identification procedures to the echinoderm fertilization assay to identify toxicity in a municipal effluent

Journal Article · Fri Dec 01 04:00:00 EST 1995 · Environmental Toxicology and Chemistry

DOI:https://doi.org/10.1002/etc.5620141223· OSTI ID:162962

Bailey, H C; Miller, J L; Miller, M J ^[1]; Dhaliwal, B S ^[2]

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1. AQUA-Science, Davis, CA (United States)
2. Central Contra Costa Sanitary District, Martinez, CA (United States)

Toxicity was detected in a municipal effluent with the echinoderm fertilization assay. Dendraster excentricus appeared more sensitive to the effluent than did Strongylocentrotus purpuratus. A Phase 1 toxicity identification evaluation (TIE) was conducted using procedures adapted to the echinoderm fertilization bioassay. The Phase 1 TIE implicated cationic metals as the cause of toxicity, and follow-up investigations suggested that copper was the primary cation responsible. As part of the TIE, bioassays were conducted on ammonia and several cations. No-observable-effect concentrations for D. excentricus were > 13.4 {micro}g/L (Ag), > 9.4 {micro}g/L (Cd), 3.8 to 13.1 {micro}g/L (Cu), > 0.7 {micro}g/L (Hg), and 10 mg/L (N, as total ammonia). The data also suggested that interspecific differences in sensitivity to copper and ammonia exist between Dendraster excentricus and Strongylocentrotus purpuratus.

• Sponsoring Organization: USDOE
• OSTI ID: 162962
• Journal Information: Environmental Toxicology and Chemistry, Journal Name: Environmental Toxicology and Chemistry Journal Issue: 12 Vol. 14; ISSN 0730-7268; ISSN ETOCDK
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
AMMONIA
BIOASSAY
CADMIUM
CHEMICAL EFFLUENTS
COPPER
ECHINODERMS
GENETIC VARIABILITY
MERCURY
MUNICIPAL WASTES
SENSITIVITY
SILVER
TOXICITY