Localization and visualization of pulmonary emboli with radiolabeled fibrin-specific monoclonal antibody
- Division of Nuclear Medicine, Massachusetts General Hospital, Boston (USA)
Indium-111-labeled monoclonal antibody 64C5 specific for the beta-chain of fibrin monomer was used to image canine (n = 6) experimental pulmonary emboli (at least one barium-thrombin and one copper-coil induced clot per dog). Uptake of {sup 111}In-64C5 and 125I-control-DIG26-11 were compared in 10 clots (7 barium-thrombin and 3 copper-coil) identified in the lungs. There was no difference in the blood clearance of {sup 111}In-64C5 and 125I-DIG26-11. Uptake of {sup 111}In-64C5 (0.183 {plus minus} 0.105, mean %ID/g) was greater than 125I-DIG26-11 (0.024 {plus minus} 0.025) in pulmonary clots (p less than 0.001). Mean thrombus to blood ratios at 24 hr were 6.78:1 for 64C5 and 0.57:1 for DIG26-11. The clots visualized in vivo were larger (0.315 {plus minus} 0.381 g) than clots not visualized (0.089 {plus minus} 0.098). Negative images were recorded in three dogs with pulmonary emboli, injected with {sup 111}In-labeled control monoclonal antibody 3H3. These data suggest that {sup 111}In-labeled antifibrin can detect large pulmonary emboli in vivo.
- OSTI ID:
- 5594423
- Journal Information:
- Journal of Nuclear Medicine; (United States), Journal Name: Journal of Nuclear Medicine; (United States) Vol. 32:6; ISSN 0161-5505; ISSN JNMEA
- Country of Publication:
- United States
- Language:
- English
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62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BLOOD COAGULATION FACTORS
BLOOD-PLASMA CLEARANCE
CLEARANCE
COAGULANTS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DISEASES
DISTRIBUTION
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ELECTRON CAPTURE RADIOISOTOPES
FIBRIN
HEMATOLOGIC AGENTS
HEMOSTATICS
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOIMMUNODETECTION
RADIOIMMUNOSCINTIGRAPHY
RADIOISOTOPE SCANNING
RADIOISOTOPES
RESPIRATORY SYSTEM DISEASES
SCINTISCANNING
SCLEROPROTEINS
SPECIFICITY
TISSUE DISTRIBUTION
TRACER TECHNIQUES
UPTAKE
VERTEBRATES