Screening of isolates and strains of Rhizobium leguminosarum biovar trifolii for heavy metal resistance using buffered media

Journal Article · Wed Sep 01 00:00:00 EDT 1993 · Environmental Toxicology and Chemistry; (United States)

DOI:https://doi.org/10.1897/1552-8618(1993)12[1643:SOIASO]2.0.CO;2· OSTI ID:5592308

Chaudri, A M; McGrath, S P ^[1]; Giller, K E ^[2]; Angle, J S ^[3]; Chaney, R L ^[4]

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1. AFRC Inst. of Arable Crops Research, Harpenden, Herts (United Kingdom). Soil Science Dept.
2. Univ. of London, Ashford, Kent (United Kingdom). Wye College, Dept. of Biochemistry and Biological Sciences
3. Univ. of Maryland, College Park, MD (United States). Dept. of Agronomy
4. USDA Agricultural Research Service, Environmental Chemistry Lab., Beltsville, MD (United States)

The computer program GEOCHEM-PC was used to calculate the metal ion activities of Cu, Zn, Cd, and Ni in defined media amended with various metal ion buffers at pH 6.6 or 6.0 so that the lowest-observed-effect concentrations (LOECs) of these metals to isolates and strains of Rhizobium leguminosarum biovar trifolii could be determined. Strains from the U.S. Department of Agriculture (USDA) Beltsville (MD) Rhizobium culture collection were generally more tolerant of the metals than any of the isolates from the sludge-treated soil (S-isolates) and those from farm-yard manure-treated soil (F-isolates), although the S- were more metal resistant than the F-isolates. All isolates and strains, however, tolerated much larger concentrations in the buffered systems than those found in the solutions of soils from which they originated. Copper toxicity, using iminodiacetate (IDA), occurred for the F- and S-isolates and USDA strains at concentrations of 16, 47, and 430 [mu]g mL[sup [minus]1], respectively, corresponding to predicted ion activities of 0.002, 0.006, and 0.06 [mu]g ml[sup [minus]1], respectively. The Zn LOECs for the F- and S-isolates, without a buffer, occurred at concentrations of 47 and 207 [mu]g ml[sup [minus]1], respectively, corresponding to predicted ion activities of 37 and 157 [mu]g ml[sup [minus]1], respectively. No toxicity occurred when nitrilotriacetate was used for Cd up to concentrations of 356 [mu]g ml[sup [minus]1]. Nickel concentrations up to 0.6 [mu]g ml[sup [minus]1] had no effect with ethylene-bis(oxyethylenenitrilo)tetraacetate and up to 186 [mu]g ml[sup [minus]1] with IDA.

• OSTI ID: 5592308
• Journal Information: Environmental Toxicology and Chemistry; (United States), Journal Name: Environmental Toxicology and Chemistry; (United States) Vol. 12:9; ISSN 0730-7268; ISSN ETOCDK
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL VARIABILITY
BUFFERS
CADMIUM
COMPUTER CODES
COPPER
ELEMENTS
G CODES
GENETIC VARIABILITY
METALS
MICROORGANISMS
NICKEL
RHIZOBIUM
TOLERANCE
TOXICITY
TRANSITION ELEMENTS
ZINC