Contributions of nuclear magnetic resonance to renal biochemistry

Journal Article · Wed Jan 01 00:00:00 EST 1986 · Kidney Int.; (United States)

DOI:https://doi.org/10.1038/ki.1986.15· OSTI ID:5590634

Ross, B; Freeman, D; Chan, L

/sup 31/P NMR as a descriptive technique is of interest to nephrologists. Particular contributions of /sup 31/P NMR to our understanding of renal function may be enumerated.: Free metabolite levels are different from those classically accepted; in particular, ADP and Pi are low with implications for the control of renal metabolism and Pi transport, and, via the phosphorylation potential, for Na+ transport. Renal pH is heterogeneous; between cortex, outer medulla, and papilla, and between cell and lumen, a large pH gradient exists. Also, quantitation between cytosol and mitochondrion of the pH gradient is now feasible. In acute renal failure of either ischemic or nonischemic origin, both ATP depletion and acidification of the renal cell result in damage, with increasing evidence for the importance of the latter. Measurements of renal metabolic rate in vivo suggest the existence of a prodromal phase of acute renal failure, which could lead to its detection at an earlier and possibly reversible stage. Human renal cancers show a unique /sup 31/P NMR spectrum and a very acidic environment. Cancer chemotherapy may alter this and detection of such changes with NMR offers a method of therapeutic monitoring with significance beyond nephrology. Renal cortex and medulla have a different T1 relaxation time, possibly due to differences in lipid composition. It seems that NMR spectroscopy has much to offer to the future understanding of the relationship between renal biochemistry and function. 56 references.

• Research Organization: Univ. of Oxford, England
• OSTI ID: 5590634
• Journal Information: Kidney Int.; (United States), Vol. 1
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
KIDNEYS
DYNAMIC FUNCTION STUDIES
UROGENITAL SYSTEM DISEASES
DIAGNOSIS
ATP
EPITHELIUM
HYPOTENSION
IN VITRO
ISCHEMIA
MEMBRANE TRANSPORT
METABOLITES
NUCLEAR MAGNETIC RESONANCE
PHOSPHORUS 31
PROTONS
RABBITS
RATS
SODIUM
ALKALI METALS
ANIMAL TISSUES
ANIMALS
BARYONS
BODY
CARDIOVASCULAR DISEASES
DISEASES
ELEMENTARY PARTICLES
ELEMENTS
FERMIONS
HADRONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
METALS
NUCLEI
NUCLEONS
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
RESONANCE
RODENTS
STABLE ISOTOPES
TISSUES
VASCULAR DISEASES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics