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Induced recovery from near- and far-UV damage in cultured marsupial cells

Thesis/Dissertation ·
OSTI ID:5585630
Colony-forming ability of cultured rat kangaroo cells (Ptk-2) decreases with increasing exposure to light from a daylight fluorescent lamp. After reaching a minimum, survival increases with further exposure, forming a V-shaped survival curve. This increase is inhibited by low concentrations of the protein synthesis inhibitor cycloheximide. The resulting V-shaped survival curve was characterized with respect to several experimental parameters; it appears to be due to an induced repair system dependent on protein synthesis. These results suggest that induced repair, dependent on protein synthesis, in response to fluorescent, near UV, and far UV light damage, is a major repair pathway in Ptk-2 cells, and provides a characterizable system that can elucidate induced repair mechanisms in other mammalian cells.
Research Organization:
Texas Univ., Dallas (USA)
OSTI ID:
5585630
Country of Publication:
United States
Language:
English