Identification of tyrosine O-sulfate in proteins by reverse-phase high-performance liquid chromatography: use of base hydrolysis combined with precolumn derivatization using phenyl isothiocyanate

Journal Article · Mon Mar 31 23:00:00 EST 1986 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(86)90500-2· OSTI ID:5585241

Christie, D L; Hill, R M; Isakow, K; Barling, P M

A procedure has been developed for the analysis of tyrosine O-sulfate in proteins. Samples are subjected to base hydrolysis with Ba(OH)/sub 2/, neutralized with sulfuric acid, and the majority of other amino acids removed by chromatography on Dowex AG 50 x 8. The average recovery of tyrosine O-sulfate from these procedures was 43%. Tyrosine O-sulfate was identified by reverse-phase HPLC as the phenylthiocarbamyl derivative following precolumn derivatization with phenyl isothiocyanate. The method has been applied to bovine fibrinogen giving a tyrosine O-sulfate content ranging from 0.59 to 1.23 mol/mol. These procedures were also shown to be suitable for the analysis of the incorporation of (/sup 35/S)sulfate into tyrosine O-sulfate residues in proteins by intact cells.

• Research Organization: Univ. of Auckland, New Zealand
• OSTI ID: 5585241
• Journal Information: Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 154:1; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
550201 -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METAL COMPOUNDS
AMINO ACIDS
ANIMALS
BARIUM COMPOUNDS
BARIUM HYDROXIDES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD COAGULATION FACTORS
CARBOXYLIC ACIDS
CATTLE
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHROMATOGRAPHY
COAGULANTS
DATA
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DOMESTIC ANIMALS
DRUGS
ESTERS
EVEN-ODD NUCLEI
EXPERIMENTAL DATA
FIBRINOGEN
GLOBULINS
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROGEN COMPOUNDS
HYDROLYSIS
HYDROXIDES
HYDROXY ACIDS
INFORMATION
INORGANIC ACIDS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
LYSIS
MAMMALS
NUCLEI
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
QUANTITY RATIO
RADIOISOTOPES
RUMINANTS
SEPARATION PROCESSES
SOLVOLYSIS
SULFONIC ACID ESTERS
SULFUR 35
SULFUR ISOTOPES
SULFURIC ACID
TRACER TECHNIQUES
TYROSINE
VERTEBRATES