Temporal pattern of incorporation of /sup 3/H precursors into pituitary glycoproteins and their subsequent release

Journal Article · Thu Apr 01 04:00:00 EST 1982 · Proc. Soc. Exp. Biol. Med.; (United States)

DOI:https://doi.org/10.3181/00379727-169-41374· OSTI ID:5580632

Grotjan, Jr, H E

The temporal pattern of incorporation of various /sup 3/H precursors into glycoproteins by rat anterior pituitaries incubated in vitro and the release of /sup 3/H-glycoproteins was examined. (/sup 3/H)Leucine incorporation was linear with respect to time and (/sup 3/H)leucine-containing macromolecules appeared in the media in about 1 hr. The temporal pattern of (/sup 3/H)mannose incorporation and release was similar. (/sup 3/H)Galactose and (/sup 3/H)fucose were incorporated after apparent time of delays of approximately 15 min and soon thereafter (20-25 min) appeared in the medium in /sup 3/H-glycoproteins. Thus, these precursors appear to be added as terminal residues. (/sup 3/H)Glucosamine exhibited a pattern intermediate between (/sup 3/H)leucine and (/sup 3/H)fucose whereas (/sup 3/H)GlcNAc appeared to be incorporated as a terminal residue.

• Research Organization: Univ. of Texas Medical School, Houston
• OSTI ID: 5580632
• Journal Information: Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 169:4; ISSN PSEBA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
ENDOCRINE GLANDS
GLANDS
GLUCOPROTEINS
HEXOSES
IN VITRO
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUCINE
MAMMALS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PITUITARY GLAND
PRECURSOR
PROTEINS
RATS
RODENTS
SACCHARIDES
SYNTHESIS
TIME DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES