Alterations in the metabolism of benzo(a)pyrene in syrian hamster embryo (SHE) cells pretreated with phenolic antioxidants
Inhibition of chemical- or raddiation-induced neoplasia has been observed in animals whose diets were supplemented with antioxidants commonly used as food additives. Inhibition of the carcinogenicity of benzo(a)pyrene (BaP) or of 7,12-dimenthylbenz(a)anthracene (DMBA) - in rats has been achieved by the addition of the phenolic antioxidants butylated hydroxyanisole (BHA) or butylated hydroxytoluene (BHT) to the diet. Our data suggest that in SHE cells antioxidants inhibit the overall metabolism of BaP to its various oxidized moieties including 7,8-diol- and 7,8,9,10-tetrol-BaP. A plausible explanation for our results with SHE cells is that the antioxidants interact directly with AHH, thus inhibiting AHH metabolic capacity. From analysis of nuclear material from SHE cells (+- antioxidants) incubated for 36 hours with BaP at 1 ..mu..g/ml, it is calculated that 4.6, 2.4 and 2.9 pmol BaP are bound to the DNA isolated from 10/sup 7/ nuclei of control, BHA-(20 ..mu..g/ml) and p-MP-(10 ..mu..g/ml) treated cultures, respectively.
- Research Organization:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 5578308
- Report Number(s):
- LA-UR-83-3093; CONF-8310179-2; ON: DE84001723
- Resource Relation:
- Conference: 8. international symposium on polynuclear aromatic hydrocarbons, Columbus, OH, USA, 26 Oct 1983
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BENZOPYRENE
METABOLISM
METABOLITES
DNA ADDUCTS
ULTRACENTRIFUGATION
FOOD
ADDITIVES
LIQUID COLUMN CHROMATOGRAPHY
ANTIOXIDANTS
CELL CULTURES
CHO CELLS
EXPERIMENTAL DATA
HAMSTERS
MIXED-FUNCTION OXIDASES
PHENOLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
X RADIATION
ADDUCTS
ANIMAL CELLS
ANIMALS
AROMATICS
CENTRIFUGATION
CHROMATOGRAPHY
CONDENSED AROMATICS
DATA
ELECTROMAGNETIC RADIATION
ENZYMES
HYDROCARBONS
HYDROXY COMPOUNDS
INFORMATION
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NUMERICAL DATA
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
RADIATIONS
RODENTS
SEPARATION PROCESSES
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
550201 - Biochemistry- Tracer Techniques