Autologous platelet-labeling in thrombocytopenia

Journal Article · Thu Nov 01 04:00:00 EST 1990 · Thrombosis Research; (USA)

DOI:https://doi.org/10.1016/0049-3848(90)90183-D· OSTI ID:5575620

Sinzinger, H; Virgolini, I; Vinazzer, H ^[1]

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1. Univ. of Vienna (Austria)

Field studies performed with peripheral platelets obtained from 6 male volunteers aged 23 to 29 years revealed an extraordinary dependence of labeling efficiency on incubation time and platelet concentration after {sup 111}In-oxine platelet labeling. Since the monitoring of in vivo-platelet function in patients with thrombocytopenia may cause problems due to insufficient labeling results and homologous platelets may show a different in vivo behaviour to autologous ones, we have searched for the minimal amount of platelets necessary to allow appropriate labeling and imaging in patients with thrombocytopenia. In 15 patients with untreated thrombocytopenia aged 14 to 79 years demonstrating a mean peripheral platelet count of 2.509 +/- 1.45 x 10(4) cells/microliters autologous {sup 111}In-oxine platelet labeling was performed. The results indicate that approximately 1 x 10(8) (concentrated) platelets/ml are necessary to obtain an adequate labeling efficiency and recovery. This platelet concentration can be easily achieved by drawing one more Monovette of whole blood per each 5 x 10(4) platelets/microliter peripheral platelet count less than 2 x 10(5)/microliter. It is concluded, that calculation of the required number of platelets in advance, variation of the blood volume drawn and the volume of incubation buffer allow informative, qualitative and quantitative results using autologous platelets. The method presented effectively circumvents the requirement of homologous platelets for radiolabeling in thrombocytopenia.

• OSTI ID: 5575620
• Journal Information: Thrombosis Research; (USA), Journal Name: Thrombosis Research; (USA) Vol. 60:3; ISSN 0049-3848; ISSN THBRA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AROMATICS
AZAARENES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY
BODY FLUIDS
CAMERAS
CARDIOVASCULAR DISEASES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIGESTIVE SYSTEM
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
GAMMA CAMERAS
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMAGE PROCESSING
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLING
LIVER
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXINE
PATIENTS
PROCESSING
PYRIDINES
QUINOLINES
RADIOISOTOPES
SPLEEN
THROMBOSIS
VASCULAR DISEASES