skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Dissimilarities between methylene blue and cyanide on relaxation and cyclic GMP formation in endothelium-intact intrapulmonary artery caused by nitrogen oxide-containing vasodilators and acetylcholine

Abstract

The objective of the present study was to ascertain whether cyanide shares the properties of methylene blue as a selective inhibitor of vascular smooth muscle relaxation elicited by agents that stimulate the formation of cyclic GMP. Experiments were performed with endothelium-intact rings prepared from bovine intrapulmonary artery. Methylene blue, a good inhibitor of soluble guanylate cyclase, antagonized both arterial relaxation and cyclic GMP accumulation in response to sodium nitroprusside, glyceryl trinitrate, S-nitroso-N-acetylpenicillamine and acetylcholine. In contrast, cyanide inhibited only the responses to sodium nitroprusside. Increasing concentrations of methylene blue depressed resting arterial levels of cyclic GMP and caused slowly developing but marked contractions whereas cyanide was without effect. Contractile responses to phenylephrine, potassium and U46619 were potentiated by methylene blue but not by cyanide. Preincubation of dilute solutions of cyanide containing sodium nitroprusside in oxygenated Krebs' buffer at 37 degrees C for 15 min before addition to bath chambers depressed relaxation and cyclic GMP accumulation caused by sodium nitroprusside markedly. Similar treatment of glyceryl trinitrate, however, failed to alter its effects in arterial rings. A chemical inactivation of sodium nitroprusside by cyanide appears to account for the specific inhibitory action of cyanide on arterial responses to sodium nitroprusside. This studymore » indicates clearly that cyanide does not share the properties of methylene blue as an inhibitor of arterial relaxation elicited by vasodilators that stimulate cyclic GMP formation.« less

Authors:
; ; ;
Publication Date:
Research Org.:
Tulane Univ. School of Medicine, New Orleans, LA
OSTI Identifier:
5575501
Resource Type:
Journal Article
Journal Name:
J. Pharmacol. Exp. Ther.; (United States)
Additional Journal Information:
Journal Volume: 1
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CYANIDES; BIOLOGICAL EFFECTS; METHYLENE BLUE; ACETYLCHOLINE; ARTERIES; CATTLE; COMPARATIVE EVALUATIONS; ENDOTHELIUM; GUANOSINE; IN VITRO; INHIBITION; MUSCLES; NITROGEN OXIDES; PHOSPHATES; VASODILATION; AMINES; AMMONIUM COMPOUNDS; ANIMAL TISSUES; ANIMALS; ANTI-INFECTIVE AGENTS; ANTIMICROBIAL AGENTS; AROMATICS; AUTONOMIC NERVOUS SYSTEM AGENTS; AZAARENES; AZINES; BLOOD VESSELS; BODY; CARDIOVASCULAR SYSTEM; CHALCOGENIDES; CHLORIDES; CHLORINE COMPOUNDS; DOMESTIC ANIMALS; DRUGS; ESTERS; HALIDES; HALOGEN COMPOUNDS; HETEROCYCLIC COMPOUNDS; MAMMALS; NEUROREGULATORS; NITROGEN COMPOUNDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; ORGANS; OXIDES; OXYGEN COMPOUNDS; PARASYMPATHOMIMETICS; PHENOTHIAZINES; PHOSPHORUS COMPOUNDS; PURINES; QUATERNARY COMPOUNDS; RIBOSIDES; RUMINANTS; TISSUES; VERTEBRATES; 560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)

Citation Formats

Ignarro, L J, Harbison, R G, Wood, K S, and Kadowitz, P J. Dissimilarities between methylene blue and cyanide on relaxation and cyclic GMP formation in endothelium-intact intrapulmonary artery caused by nitrogen oxide-containing vasodilators and acetylcholine. United States: N. p., 1986. Web.
Ignarro, L J, Harbison, R G, Wood, K S, & Kadowitz, P J. Dissimilarities between methylene blue and cyanide on relaxation and cyclic GMP formation in endothelium-intact intrapulmonary artery caused by nitrogen oxide-containing vasodilators and acetylcholine. United States.
Ignarro, L J, Harbison, R G, Wood, K S, and Kadowitz, P J. 1986. "Dissimilarities between methylene blue and cyanide on relaxation and cyclic GMP formation in endothelium-intact intrapulmonary artery caused by nitrogen oxide-containing vasodilators and acetylcholine". United States.
@article{osti_5575501,
title = {Dissimilarities between methylene blue and cyanide on relaxation and cyclic GMP formation in endothelium-intact intrapulmonary artery caused by nitrogen oxide-containing vasodilators and acetylcholine},
author = {Ignarro, L J and Harbison, R G and Wood, K S and Kadowitz, P J},
abstractNote = {The objective of the present study was to ascertain whether cyanide shares the properties of methylene blue as a selective inhibitor of vascular smooth muscle relaxation elicited by agents that stimulate the formation of cyclic GMP. Experiments were performed with endothelium-intact rings prepared from bovine intrapulmonary artery. Methylene blue, a good inhibitor of soluble guanylate cyclase, antagonized both arterial relaxation and cyclic GMP accumulation in response to sodium nitroprusside, glyceryl trinitrate, S-nitroso-N-acetylpenicillamine and acetylcholine. In contrast, cyanide inhibited only the responses to sodium nitroprusside. Increasing concentrations of methylene blue depressed resting arterial levels of cyclic GMP and caused slowly developing but marked contractions whereas cyanide was without effect. Contractile responses to phenylephrine, potassium and U46619 were potentiated by methylene blue but not by cyanide. Preincubation of dilute solutions of cyanide containing sodium nitroprusside in oxygenated Krebs' buffer at 37 degrees C for 15 min before addition to bath chambers depressed relaxation and cyclic GMP accumulation caused by sodium nitroprusside markedly. Similar treatment of glyceryl trinitrate, however, failed to alter its effects in arterial rings. A chemical inactivation of sodium nitroprusside by cyanide appears to account for the specific inhibitory action of cyanide on arterial responses to sodium nitroprusside. This study indicates clearly that cyanide does not share the properties of methylene blue as an inhibitor of arterial relaxation elicited by vasodilators that stimulate cyclic GMP formation.},
doi = {},
url = {https://www.osti.gov/biblio/5575501}, journal = {J. Pharmacol. Exp. Ther.; (United States)},
number = ,
volume = 1,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1986},
month = {Wed Jan 01 00:00:00 EST 1986}
}