High-performance liquid chromatographic analysis of 2-aminofluorene and its metabolites in monolayer cultures of rat hepatocytes
A high-pressure liquid chromatography method was developed to separate 2-aminofluorene and several ring-hyroxylated metabolites. The acetylated derivatives of these compounds and N-hydroxy-2-acetylaminofluorene were also separated simultaneously. HPLC analyses were performed using a Dupont Zorbax C-8 HPLC column and a solvent mixture of 0.02 M acetic acid and isopropanol. Isopropanol concentrations were increased from 27 to 100% over 45 min using a concave gradient system. Desferal mesylate was added to the aqueous component to improve the resolution of several hydroxylated arylamine metabolites. The method was used to quantitate the metabolites of 2-aminofluorene in monolayer cultures of rat hepatocytes.
- Research Organization:
- American Health Foundation, Valhalla, NY
- OSTI ID:
- 5569889
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 153:1; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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400102* -- Chemical & Spectral Procedures
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59 BASIC BIOLOGICAL SCIENCES
ACETYLATION
ACYLATION
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
CARCINOGENS
CELL CULTURES
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHROMATOGRAPHY
CONDENSED AROMATICS
EXTRACTION
FLUORENE
HYDROCARBONS
HYDROXYLATION
LIQUID COLUMN CHROMATOGRAPHY
LIVER CELLS
MAMMALS
METABOLITES
ORGANIC COMPOUNDS
POLYCYCLIC AROMATIC AMINES
QUANTITATIVE CHEMICAL ANALYSIS
RATS
RODENTS
SEPARATION PROCESSES
SOLVENT EXTRACTION
SOMATIC CELLS
VERTEBRATES