Molecular biology and physiology of methanogenic archaebacteria. Annual report, July 1988-June 1989
Methane-producing archaebacteria are worthy of their novel biology and potential in anaerobic bioprocessing. This work continues to study the biochemistry, genetics, and molecular biology of the thermophilic autotroph Methanobacterium thermoautotrophicum. DNA from antimetabolite-resistant mutant strains was used to transform sensitive recipient cells to resistance, and DNA was cloned into Escherichia coli plasmids. This DNA will be mutated with transposons in the E. coli host, then isolated and used to transform methanogen cells to selectable mutant phenotypes. Mutant strains resistant to purine analogs were used to determine that wild type cells of M. thermoautotrophicum possess an almost complete set of enzymes for uptake, activation, and interconversion of purine bases and nucleosides. These mutants and the information about the pathways will be the basis for generating a genetic map. Metabolic studies of a unique formate auxotroph revealed a new role for this one carbon compound in the anabolic metabolism of this methanogen.
- Research Organization:
- Oklahoma Univ., Norman, OK (USA). Dept. of Botany and Microbiology
- OSTI ID:
- 5566321
- Report Number(s):
- AD-A-210399/2/XAB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANABOLISM
AROMATICS
AZAARENES
BACTERIA
BIOCHEMISTRY
BIOLOGY
CARBON
CARBOXYLIC ACID SALTS
CELL CONSTITUENTS
CHEMISTRY
CHROMOSOMES
CLONING
DNA
DOCUMENT TYPES
ELEMENTS
ENZYMES
ESCHERICHIA COLI
FORMATES
GENES
GENETIC MAPPING
GENETICS
HETEROCYCLIC COMPOUNDS
MAPPING
METABOLISM
METHANOGENIC BACTERIA
MICROORGANISMS
MOLECULAR BIOLOGY
MUTANTS
NONMETALS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENOTYPE
PHYSIOLOGY
PLASMIDS
PROGRESS REPORT
PURINES
RIBOSIDES
TRANSPOSONS