Poly(deoxyadenylic-deoxythymidylic acid) damage by radiolytically activated neocarzinostatin
Journal Article
·
· Biochemistry; (United States)
The anaerobic reaction of poly(deoxyadenylic-deoxythymidylic acid) with neocarzinostatin activated by the carboxyl radical CO/sub 2/-, an electron donor generated from gamma-ray radiolysis of nitrous oxide saturated formate buffer, has been characterized. DNA damage includes base release and strand breaks. Few strand breaks are formed prior to alkaline treatment; they bear 3'-phosphoryl termini. In contrast, most (66%) of the base release occurs spontaneously. DNA damage is highly (95%) specific for thymidine sites. Neither DNA-drug covalent adduct nor nucleoside 5'-aldehyde, which are major products in the DNA-nicking reaction initiated by mercaptans and oxygen, is formed in this reaction. Data are presented to show that the CO/sub 2/(-)-activated neocarzinostatin intermediate is a short-lived free radical able to abstract hydrogen atoms from the C-1' and C-5' positions of deoxyribose. Attack occurs mostly (68%) at the C-1' position, producing a lesion whose properties are consistent with those of (oxidized) apyrimidinic sites.
- Research Organization:
- Harvard Medical School, Boston, MA
- OSTI ID:
- 5565564
- Journal Information:
- Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 24:2; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Mechanism and base specificity of DNA breakage in intact cells by neocarzinostatin
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Journal Article
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Mon Jan 26 23:00:00 EST 1987
· Biochemistry; (United States)
·
OSTI ID:6620117
Incorporation of /sup 18/O/sub 2/ into thymidine 5'-aldehyde in neocarzinostatin chromophore-damaged DNA
Journal Article
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Sat Aug 25 00:00:00 EDT 1984
· J. Biol. Chem.; (United States)
·
OSTI ID:5332023
Effect of apurinic/apyrimidinic endonucleases and polyamines on DNA treated with bleomycin and neocarzinostatin: specific formation and cleavage of closely opposed lesions in complementary strands
Journal Article
·
Tue May 17 00:00:00 EDT 1988
· Biochemistry; (United States)
·
OSTI ID:7024779
Related Subjects
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTINEOPLASTIC DRUGS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
COVALENCE
DECOMPOSITION
DNA
DNA ADDUCTS
DRUGS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
NEOCARCINOSTATIN
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RADICALS
RADIOLYSIS
RADIOMIMETIC DRUGS
RIBOSIDES
STRAND BREAKS
THYMIDINE
THYMIDYLIC ACID
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTINEOPLASTIC DRUGS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
COVALENCE
DECOMPOSITION
DNA
DNA ADDUCTS
DRUGS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
NEOCARCINOSTATIN
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RADICALS
RADIOLYSIS
RADIOMIMETIC DRUGS
RIBOSIDES
STRAND BREAKS
THYMIDINE
THYMIDYLIC ACID