Sister chromatid exchange (SCE) induced by laser-UV- microirradiation: correlation between the distribution of photolesions and the distribution of SCEs

Journal Article · Sat Dec 31 23:00:00 EST 1983 · Basic Life Sci.; (United States)

DOI:https://doi.org/10.1007/978-1-4684-4889-4_15· OSTI ID:5565417

Raith, M; Cremer, T; Cremer, C; Speit, G

Small, medium, and large nuclear areas comprising approximately 5, 30, or 80% of the total area of the interphase nuclei of Chinese hamster cells (M3-1) cultivated in vitro were irradiated with a laser-UV- microbeam of wavelength 257 nm. The DNA of the cells was substituted with 5-bromodeoxyuridine (BrdUrd) for 1 cell cycle in one set of experiments. After microirradiation the cells were grown for a second cycle in medium without BrdUrd (protocol A). In a second set, cells with nonsubstituted DNA were microirradiated and grown for 2 additional cycles, the first in the presence, the second in the absence of BrdUrd (protocol B). In situ chromosome preparation and differential chromatid staining was subsequently performed. The induction of sister chromatid exchanges (SCEs) was found to be dependent on both the ultraviolet (UV) dose and the spatial distribution of the UV energy within the cell nucleus. Following both protocols the average number of chromosomes with SCEs was significantly higher after microirradiation of a large nuclear area as compared to microirradiation of a small nuclear area. In the latter case, multiple SCEs were noted on individual chromosome arms at the first postirradiation mitosis (protocol A). In other cells, especially at higher doses, protocol A resulted in shattering of a few closely neighbored chromosomes which were surrounded by intact ones with normal SCE levels. Microirradiation of medium-sized nuclear areas produced high levels of SCEs over a number of chromosomes which still appeared spatially related in a part of the metaphase spread.

• Research Organization: Universitaet Heidelberg Im Neuenheimer Feld, Federal Republic of Germany
• OSTI ID: 5565417
• Journal Information: Basic Life Sci.; (United States), Journal Name: Basic Life Sci.; (United States); ISSN BLFSB
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTIMETABOLITES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL DIVISION
CHO CELLS
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
DEOXYURIDINE
DNA
DRUGS
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INJURIES
IRRADIATION
LASERS
MITOSIS
MUTATIONS
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATION INJURIES
RADIATIONS
RIBOSIDES
SISTER CHROMATID EXCHANGES
ULTRAVIOLET RADIATION
URACILS