Characterization of specific binding sites for PAF in the iris and ciliary body of rabbit

Journal Article · Fri Apr 14 04:00:00 EDT 1989 · Biochemical and Biophysical Research Communications; (USA)

DOI:https://doi.org/10.1016/0006-291X(89)91648-3· OSTI ID:5564206

Domingo, M T; Chabrier, P E; Van Delft, J L; Verbeij, N L; Van Haeringen, N J; Braquet, P ^[1]

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1. Institut Henri Beaufour LES ULIS (France)

The protective effect exerted by BN 52021 a specific PAF-receptor antagonist in experimentally induced ocular inflammatory disorders led us to investigate the possible presence of specific receptors for PAF in rabbit iris and ciliary body. Two classes of PAF binding sites were found in isolated iris and ciliary process of pigmented rabbit eyes: a high affinity site Kd1 congruent to 4.9 +/- 0.47 nM, Bmax1 congruent to 3.17 +/- 0.50 pmoles/mg protein, a low affinity sites Kd2 congruent to 11.6 +/- 0.33 nM, Bmax2 congruent to 12.46 +/- 2.3 pmoles/mg protein for iris. The specific binding was not affected by lyso-PAF the biologically inactive precursor and metabolite of PAF, up to 10(-6) M; inhibition by unlabelled PAF demonstrated a biphasic curve partially antagonized by BN 52021. The present results demonstrate the presence of specific binding sites for PAF in rabbit eyes which could mediate the action of this mediator in eye inflammatory processes and explain the protective effect observed with BN 52021.

• OSTI ID: 5564206
• Journal Information: Biochemical and Biophysical Research Communications; (USA), Journal Name: Biochemical and Biophysical Research Communications; (USA) Vol. 160:1; ISSN BBRCA; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BLOOD COAGULATION FACTORS
BODY
BODY AREAS
CHEMICAL COMPOSITION
COAGULANTS
DISEASES
DRUGS
ESTERS
EYES
FACE
HEAD
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INFLAMMATION
ISOTOPE APPLICATIONS
KINETICS
LACTONES
MAMMALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PROTEINS
RABBITS
REACTION KINETICS
RECEPTORS
SENSE ORGANS
SENSE ORGANS DISEASES
SYMPTOMS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES