Characterization of two dissimilatory sulfite reductases (desulforubidin and desulfoviridin) from the sulfate-reducing bacteria. Moessbauer and EPR studies

Journal Article · Wed Feb 17 04:00:00 EST 1988 · J. Am. Chem. Soc.; (United States)

DOI:https://doi.org/10.1021/ja00212a013· OSTI ID:5563249

Moura, I; LeGall, J; Lino, A R; Peck, Jr, H D; Fauque, G; Xavier, A V; DerVartanian, D V; Moura, J J.G.; Huynh, B H

In this paper, the authors report a detailed Moessbauer investigation of two different sulfite reductases, namely, desulforubidin from D. baculatus and desulfoviridin from D. gigas. In order to better characterize the prosthetic groups, they have also studied the EPR spectra and determined the iron and heme contents of the /sup 57/Fe-enriched enzymes. They found that desulforubidin contains exchange-coupled siroheme-(4Fe-4S) units which are similar to those found in the hemoprotein subunit of E. coli sulfite reductase. To their surprise, they discovered that the majority of the purified desulfoviridin contains demetalized sirohydrochlorin, with only a minor portion of the sample containing siroheme. The siroheme in desulfoviridin was also found to be coupled with a (4Fe-4S) cluster.

• Research Organization: Emory Univ., Atlanta, GA
• DOE Contract Number: AS09-79ER10499
• OSTI ID: 5563249
• Journal Information: J. Am. Chem. Soc.; (United States), Journal Name: J. Am. Chem. Soc.; (United States) Vol. 110:4; ISSN JACSA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION SPECTROSCOPY
BACTERIA
CHEMICAL REACTIONS
DATA
DESULFOVIBRIO
ELECTRON SPIN RESONANCE
ENZYMES
EXPERIMENTAL DATA
GAMMA SPECTROMETERS
INFORMATION
MAGNETIC RESONANCE
MEASURING INSTRUMENTS
MICROORGANISMS
MOESSBAUER SPECTROMETERS
NUMERICAL DATA
OXIDOREDUCTASES
REDUCTION
RESONANCE
SPECTROMETERS
SPECTROSCOPY
SULFATE-REDUCING BACTERIA