Delayed excretion of 3-methylhistidine in goats

Journal Article · Tue Dec 01 04:00:00 EST 1987 · J. Nutr.; (United States)

OSTI ID:5560788

Brown, D L; Barnes, D M; Calvert, C C

The purpose of this study was to evaluate the use of 3-methylhistidine (3MH) as an index of muscle protein degradation in dairy goats. The criterion for validation was the rapid and quantitative excretion of radiolabeled 3MH. Three adult dry does and four bucks were confined to metabolism cages and injected with 5 ml of L-3-(/sup 14/C)methylhistidine dihydrochloride (4 microCi/mL) diluted in normal saline. The does' urine was collected from catheters and the bucks' urine from stainless steel separators protected with fecal collection bags. The daily urine samples were analyzed for labeled amino acid. Urinary recovery of radioactivity from all does was less than 33% after 3 d and was proceeding at less than 5% d. After 9 d, total recovery was less than 50% of total dose for the does. Elimination rates were slightly higher and more variable for the yearling bucks (25-63% after 4 d). On the basis of these data, 3MH does not appear to be a valid index of muscle protein degradation in either male or female dairy goats.

• Research Organization: Univ. of California, Davis
• OSTI ID: 5560788
• Journal Information: J. Nutr.; (United States), Journal Name: J. Nutr.; (United States) Vol. 117:12; ISSN JONUA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
AZOLES
BIOLOGICAL INDICATORS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CLEARANCE
DOMESTIC ANIMALS
EXCRETION
GOATS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTIDINE
IMIDAZOLES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
MUSCLES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
RUMINANTS
TRACER TECHNIQUES
VERTEBRATES