Inactivation of aflatoxin B1 by using the synergistic effect of hydrogen peroxide and gamma radiation

Journal Article · Tue Jan 31 23:00:00 EST 1989 · Applied and Environmental Microbiology; (USA)

OSTI ID:5560721

Patel, U D; Govindarajan, P; Dave, P J ^[1]

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1. M.S. Univ. of Baroda (India)

Inactivation of aflatoxin B1 was studied by using gamma radiation and hydrogen peroxide. A 100-krad dose of gamma radiation was sufficient to inactivate 50 micrograms of aflatoxin B1 in the presence of 5% hydrogen peroxide, and 400 krad was required for total degradation of 100 micrograms of aflatoxin in the same system. Degradation of aflatoxin B1 was confirmed by high-pressure liquid chromatographic and thin-layer chromatographic analysis. Ames microsomal mutagenicity test showed loss of aflatoxin activity. This method of detoxification also reduces the toxin levels effectively in artificially contaminated groundnuts.

• OSTI ID: 5560721
• Journal Information: Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 55:2; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AFLATOXIN
ASPERGILLUS
BACTERIA
CHEMICAL RADIATION EFFECTS
CHEMISTRY
CHROMATOGRAPHY
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EUMYCOTA
FUNGI
GAMMA RADIATION
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
IONIZING RADIATIONS
LIQUID COLUMN CHROMATOGRAPHY
MICROORGANISMS
MUTAGEN SCREENING
OXYGEN COMPOUNDS
PEROXIDES
PLANTS
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
SEPARATION PROCESSES
SYNERGISM
TOXICITY