Sequential sup 1 H NMR assignments of kistrin, a potent platelet aggregation inhibitor and glycoprotein IIb-IIIa antagonist

Journal Article · Tue Feb 04 04:00:00 EST 1992 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00119a011· OSTI ID:5559832

Adler, M; Wagner, G ^[1]

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1. Harvard Medical School, Boston, MA (United States)

Sequence-specific nuclear magnetic resonances assignments have been obtained for the protons of kistrin. Kistrin is a small naturally occurring snake venom protein that inhibits platelet aggregation by blocking the interaction of fibrinogen with the membrane-bound glycoprotein IIb-IIIa (GP IIb-IIIa), a receptor from the integrin family. Kistrin has an Arg-Gly-Asp sequence which is believed to form an adhesion recognition sequence that is essential for activity. Therefore, the interaction between kistrin and GP IIb-IIIa may provide important information on the motif used by integrins to recognize their target proteins which bear RGD sequences. Kistrin consists of 68 residues and contains six intramolecular disulfide bonds. Although one-third of the amide protons are protected from exchange with the solvent, there appears to be little or no regular secondary structure. The large number of NOE's between residues separated by two and three positions in the sequence indicates that the protein contains a large number of tightly packed loops. Along with the sequential assignments, this paper also discusses the construction and use of computerized data bases for manipulating NMR results. A strategy for computer-assisted sequential resonance using these data bases is also presented.

• OSTI ID: 5559832
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 31:4; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
ARGININE
ASPARTIC ACID
BARYONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION
BLOOD COAGULATION FACTORS
BLOOD PLATELETS
BODY FLUIDS
CARBOXYLIC ACIDS
COAGULANTS
COMPUTER CALCULATIONS
DRUGS
ELEMENTARY PARTICLES
FERMIONS
GLYCINE
GLYCOPROTEINS
HADRONS
HEMATOLOGIC AGENTS
INHIBITION
MAGNETIC RESONANCE
MATERIALS
NUCLEAR MAGNETIC RESONANCE
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
PROTONS
REPTILES
RESONANCE
SNAKES
VENOMS
VERTEBRATES