Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Hypothermic potentiation of radiation lethality in HeLa cells. [Low temperature correction factors for interpreting repair data for potentially lethal and sublethal damage]

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3575024· OSTI ID:5558129
Incubation of HeLa cells at room temperature after, but not before, irradiation enhances lethality in irradiated cells. Incubation at room temperature between split doses of irradiation decreases recovery from sublethal damage, when appropriate corrections are introduced. Without such corrections, an increase in survival is seen in cells irradiated with split doses when incubation at suboptimal temperatures extends to similar periods irrespective of the mode of irradiation; such an increase could be traced to unequal potentiation of lethality in cells irradiated with a single (full) and a half dose. It is concluded that introduction of appropriate controls and corrections is essential for the separation of repair of potentially lethal radiation damage from repair of sublethal damage.
Research Organization:
Memorial Sloan-Kettering Cancer Center, New York, NY
OSTI ID:
5558129
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 19:1; ISSN RAREA
Country of Publication:
United States
Language:
English