Animal model for evaluation of topical photoprotection against ultraviolet A (320-380 nm) radiation

Journal Article · Thu Oct 01 00:00:00 EDT 1987 · J. Invest. Dermatol.; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12471776· OSTI ID:5556874

Chew, S; DeLeo, V A; Harber, L C

Recent studies reporting UVA (ultraviolet A radiation 320-380 nm) as an integral part of the cumulative sun-induced damage in human skin have prompted an interest in developing effective UVA photoprotective agents. The development of such compounds has been impeded by the absence of a clinically relevant animal model for evaluating their efficacy. This report describes the development and use of such a laboratory animal system. Selected concentrations of oxybenzone (2-hydroxy-4-methoxybenzophenone) in vehicle (0.1% to 6%) or vehicle alone were applied to the depilated dorsal skin of 30 Hartley strain female albino guinea pigs. The skin was irradiated with solar simulated UVA from a xenon light source. Acute radiation-induced damage was assayed by erythema grading and inhibition of (/sup 3/H)thymidine incorporation into epidermal DNA. Data from erythema grading studies indicated that a significant degree of photoprotection was achieved with 6%, 3%, and 1% solutions of benzophenone compared with the control vehicle; the 6% solution was significantly more photoprotective than the 3% and 1% solutions. A 6% solution afforded significant photoprotection when assayed by (/sup 3/H)thymidine incorporation.

• Research Organization: Columbia Univ., New York, NY
• OSTI ID: 5556874
• Journal Information: J. Invest. Dermatol.; (United States), Vol. 89:4
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
RADIOPROTECTIVE SUBSTANCES
EVALUATION
SKIN
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL MODELS
DNA
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
ERYTHEMA
GUINEA PIGS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BODY
DRUGS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RIBOSIDES
RODENTS
SYMPTOMS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals
550201 - Biochemistry- Tracer Techniques