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Autoradiographic localization of /sup 3/H-digoxin binding by neural cells in the medulla

Journal Article · · Anat. Rec.; (United States)
; ;
The purpose of this investigation was to localize binding sites for the cardiac glycoside digoxin in the medulla of the rat in vivo. Adult male Sprague-Dawley rats were injected (IV) with /sup 3/H-digoxin and killed 30 minutes later. Autoradiographs of medullas showed evidence of /sup 3/H-digoxin binding to small- and medium-sized neural cells in the regions of the nucleus solitarius, dorsal motor nucleus of the vagus, area postrema, and in the zone between the area postrema and the underlying neuropil. However, the parasympathetic preganglionic neurons of the dorsal motor nucleus were not labeled. The /sup 3/H-digoxin-labeled cells in the medulla were located mainly in the commissural and medial portions of nucleus solitarius at the level of the area postrema. Animals injected with unlabeled digoxin followed by /sup 3/H-digoxin showed reduced binding of radioactivity. The small- and medium-sized neurons of the caudal portions of the nucleus solitarius are internuncial in position with respect to cardiovascular afferents of the glossopharyngeal and vagus nerves and sympathetic and parasympathetic cardiovascular efferent neurons of the medulla. The results of this study suggest that these /sup 3/H-digoxin-labeled cells, presumably neurons of nucleus solitarius, may possess high affinity binding sites for digoxin. Further, the area postrema, which lacks a blood-brain barrier, may provide a portal of entry for /sup 3/H-digoxin into regions of the medulla known to contain neurons that play a role in the regulation of cardiac rhythm.
Research Organization:
Univ. of Kentucky College of Medicine, Lexington
OSTI ID:
5554380
Journal Information:
Anat. Rec.; (United States), Journal Name: Anat. Rec.; (United States) Vol. 211:1; ISSN ANREA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL LOCALIZATION
BODY
BRAIN
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
CHEMICAL BONDS
DIGITALIS GLYCOSIDES
DIGOXIN
DRUGS
GLYCOSIDES
IN VIVO
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NERVES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
RATS
RODENTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES