Extent of histone modifications and H1/sup 0/ content during cell cycle progression in the presence of butyrate

Journal Article · Sat Jan 01 04:00:00 EST 1983 · Exp. Cell Res.; (United States)

DOI:https://doi.org/10.1016/0014-4827(83)90222-7· OSTI ID:5553958

D'Anna, J A; Gurley, L R; Tobey, R A

The effects of butyrate upon the extents of phosphorylation of histones H1 and H1/sup 0/ during cell-cycle progression have been investigated. Chinese hamster (line CHO) cells were synchronized in early S phase and released into medium containing 0 or 15 mM butyrate to resume cell-cycle traverse into G1 of the next cell cycle. Cells were also mechanically selected from monolayer cultures grown in the presence of colcemid and 0 or 15 mM butyrate to obtain >98% pure populations of metaphase cells. Although cell cycle progression is altered by butyrate, electrophoretic patterns of histones H1, H1/sup 0/, H3, and H4 indicate that butyrate has little, if any, effect on the extents of H1 and H1/sup 0/ phosphorylation during the cell cycle or the mitotic-specific phosphorylation of histone H3. Butyrate does, however, inhibit removal of extraordinary levels of histone H4 acetylation (hyperacetylation) during metaphase, and it appears to cause an increase in the content of H1/sup 0/ in chromatin during the S or G2 phases of the cell cycle. 38 references, 4 figures, 1 table.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 5553958
• Journal Information: Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 147; ISSN ECREA
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETYLATION
ACYLATION
ANIMAL CELLS
BIOLOGICAL EFFECTS
BUTYRIC ACID
CARBOXYLIC ACIDS
CELL CULTURES
CELL CYCLE
CHEMICAL REACTIONS
CHO CELLS
DATA
ESTERS
EXPERIMENTAL DATA
HISTONES
INFORMATION
INHIBITION
MONOCARBOXYLIC ACIDS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORYLATION
PROTEINS
SYNCHRONOUS CULTURES