Histones H1/sup 0/a and H1/sup 0/b are the same as CHO histones H1(III) and H1(IV):new features of H1/sup 0/ phosphorylation during the cell cycle

Journal Article · Thu Jan 01 04:00:00 EST 1981 · Biochemistry; (United States)

OSTI ID:5245797

D'Anna, J A ^[1]; Gurley, L R; Becker, R R

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1. Los Alamos National Lab., NM

Two histone H1 fractions (H1(I) and H1(II) and two histone H1/sup 0/ fractions (H1/sup 0/a and H1/sup 0/b) have been isolated from butyrate-treated Chinese hamster (line CHO) cells by guanidine hydrochloride gradient chromatography on Bio-Rex 70 ion-exchange resin. The fractions have been identified by electrophoresis and amino acid analyses. Electrophoretic analysis of cyanogen bromide treated H1/sup 0/ in long acid-urea-polyacrylamide gels suggests that H1/sup 0/a and H1/sup 0/b differ, at least, within the 20-30 residue fragment(s) removed by the cyanogen bromide clevage. Shallow-gradient Bio-Rex 70 chromatography indicates that histones H1/sup 0/a and H1/sup 0/b are the same as the respective CHO histones, H1(III) and H1(IV). This identification and the phosphate incorporation data of Gurley et al. (1975) reveal new features about H1/sup 0/ phosphorylation: (1) following release from G/sub 1/ arrest, H1/sup 0/a and H1/sup 0/b become phosphorylated in late G/sub 1/ prior to DNA synthesis; (2) H1/sup 0/a and H1/sup 0/b are phosphorylated at similar rates throughout the cell cycle. These and other data demonstrate that histone H1/sup 0/ is phosphorylated in a cell cycle dependent fashion which mimics that of histone H1.

• OSTI ID: 5245797
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 20:15; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
CARBOXYLIC ACIDS
CELL CYCLE
CHEMICAL REACTIONS
HAMSTERS
HISTONES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORYLATION
PROTEINS
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES