The lower radiosensitivity of mouse kidney cells irradiated in vivo than in vitro: a cell contact effect phenomenon

Journal Article · Sat Jun 01 00:00:00 EDT 1991 · International Journal of Radiation Oncology, Biology and Physics; (United States)

DOI:https://doi.org/10.1016/0360-3016(91)90234-U· OSTI ID:5553835

Jen, Y M; West, C M; Hendry, J H ^[1]

---

1. Department of Radiobiology, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester (United Kingdom)

For mouse kidney cells assayed in primary culture, the Do and n values were 1.1 {plus minus} 0.06 Gy and 7 {plus minus} 2 for single cells irradiated in vitro, and 1.3 {plus minus} 0.08 Gy and 25 {plus minus} 11 for in vivo irradiation. The lower radiosensitivity in vivo was shown not to be caused by natural hypoxia, as the average oxygen enhancement ratios were 2.6 {plus minus} 0.3 for in vitro and 2.8 {plus minus} 0.4 for in vivo irradiation. Irradiations of fragments of kidney tubules produced similar survivals as irradiations of kidneys in situ, even for irradiation immediately before the fragments were disaggregated into single cells. The critical point of change in radiosensitivity from in vivo to in vitro values due to this contact effect was the time that the kidney cells were monodispersed.

• OSTI ID: 5553835
• Journal Information: International Journal of Radiation Oncology, Biology and Physics; (United States), Vol. 20:6; ISSN 0360-3016
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
TUMOR CELLS
RADIOSENSITIVITY
ANOXIA
IN VITRO
IN VIVO
MICE
RADIATION DOSES
TUBULES
ANIMAL CELLS
ANIMALS
BODY
DOSES
KIDNEYS
MAMMALS
ORGANS
RODENTS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
560152 - Radiation Effects on Animals- Animals