Segmental pancreatic allograft survival in baboons treated with combined irradiation and cyclosporine: a preliminary report

Journal Article · Sun Mar 31 23:00:00 EST 1985 · Surgery; (United States)

OSTI ID:5549343

du Toit, D F; Heydenrych, J J; Smit, B; Louw, G; Zuurmond, T; Laker, L; Els, D; Weideman, A; Wolfe-Coote, S; van der Merwe, E A

The present study was undertaken to evaluate the effectiveness of cyclosporine (CS) alone, total lymphoid irradiation (TLI) alone, and CS in combination with total body irradiation (TBI) in suppressing segmental pancreatic allograft rejection in totally pancreatectomized outbred chacma baboons. The administration of CS 25 mg/kg/day and 50 mg/ kg/day resulted in mean graft survival of 21.5 days and 24.5 days, respectively. CS 85 mg/kg/day resulted in median graft survival of 9 days. There was a wide daily fluctuation of CS serum trough levels exhibited between primates receiving the same oral dose. TBI in excess of 300 rads resulted in irreversible bone marrow suppression. Modest results were achieved in recipients of TBI-76 rads (38 x 2 rads), with median graft survival of 21 days, results not different from recipients treated with CS. TLI recipients of 600 rads (150 x 4 rads) resulted in median pancreatic graft survival of 16 days. TBI together with oral CS administration exhibited no synergistic or additive effect and a single peroperative donor-specific blood transfusion did not enhance pancreatic allograft survival in this model. However, of 10 primates receiving TBI 100 rads (50 x 2 rads) and CS 25 mg/kg/day administered orally indefinitely, four remained normoglycemic for more than 60 days. TBI 100 rads (50 x 2 rads) together with oral and parenteral CS resulted in necrotizing enterocolitis in four of six recipients.

• Research Organization: Univ. of Stellenbosch Medical School, Tygerberg, South Africa
• OSTI ID: 5549343
• Journal Information: Surgery; (United States), Journal Name: Surgery; (United States) Vol. 97:4; ISSN SURGA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
560152 -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
APES
BIOLOGICAL MODELS
BODY
BONE MARROW
DIGESTIVE SYSTEM
DOSES
ENDOCRINE GLANDS
EXTERNAL IRRADIATION
GLANDS
GRAFTS
HEMATOPOIETIC SYSTEM
IMMUNOSUPPRESSION
IRRADIATION
MAMMALS
METABOLITES
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PEPTIDES
PRIMATES
PROTEINS
RADIATION DOSES
SURVIVAL TIME
SYNERGISM
THERAPY
TISSUES
TRANSFUSIONS
TRANSPLANTS
VERTEBRATES
WHOLE-BODY IRRADIATION