Characterization of deoxyribonucleic acid from cells infected with Aleutian disease virus
Viral DNA was extracted from Crandell feline kidney (CRFK) cells infected with Aleutian disease virus (ADV) and labeled with (/sup 3/H)thymidine. The sedimentation coefficient in alkaline sucrose gradients was 16S corresponding to a molecular weight of 1.5 X 10(6). The buoyant densities of DNA from infected and control cells were determined by isopyknic sedimentation in CsCl and NaI gradients. Two additional peaks of (/sup 3/H)DNA were found in infected cells, but not in control cell extracts. Fractionation of this DNA on hydroxylapatite indicated that the new peaks represented a single-stranded component, density 1.728 g/cm3, and a double-stranded component, presumed to be a viral replicative intermediate, density 1.718 g/cm3. The target antigen formation in CRFK cells was measured by gamma-irradiation of ADV and assayed for focus formation. The calculated size of ADV based on these measurements was 1.1 X 10(6). The H-1 parvovirus also was shown to have a size of 1.5 X 10(6) daltons for both antigen and plaque formation. The data indicated similarities existed between ADV and other autonomously replicating parvoviruses in most properties, except that less-than-unit length genome of ADV may be transcribed.
- Research Organization:
- Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana
- OSTI ID:
- 5546866
- Journal Information:
- Am. J. Vet. Res.; (United States), Vol. 44:7
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
BIOSYNTHESIS
CENTRIFUGATION
LABELLING
MOLECULAR WEIGHT
SIZE
VIRUSES
COMPARATIVE EVALUATIONS
ANTIGENS
CATS
CELL CULTURES
ELECTROPHORESIS
GAMMA RADIATION
LIQUID COLUMN CHROMATOGRAPHY
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
AZINES
CHROMATOGRAPHY
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PYRIMIDINES
RADIATIONS
RIBOSIDES
SEPARATION PROCESSES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques