Transformation of human fibroblasts by ionizing radiation, a chemical carcinogen, or simian virus 40 correlates with an increase in susceptibility to the autonomous parvoviruses H-1 virus and minute virus of mice
Morphologically altered and established human fibroblasts, obtained either by /sup 60/Co gamma irradiation, treatment with the carcinogen 4-nitroquinoline 1-oxide, or simian virus 40 (SV40) infection, were compared with their normal finite-life parental strains for susceptibility to the autonomous parvoviruses H-1 virus and the prototype strain of minute virus of mice (MVMp). All transformed cells suffered greater virus-induced killing than their untransformed progenitors. The cytotoxic effect of H-1 virus was more severe than that of MVMp. Moreover, the level of viral DNA replication was much (10- to 85-fold) enhanced in the transformants compared with their untransformed parent cells. Thus, in this system, cell transformation appears to correlate with an increase in both DNA amplification and cytotoxicity of the parvoviruses. However, the accumulation of parvovirus DNA in the transformants was not always accompanied by the production of infectious virus. Like in vitro-transformed fibroblasts, a fibrosarcoma-derived cell line was sensitive to the killing effect of both H-1 virus and MVMp and amplified viral DNA to high extents. The results indicate that oncogenic transformation can be included among cellular states which modulate permissiveness to parvoviruses under defined growth conditions.
- Research Organization:
- Institut National de la Sante et de la Recherche Meedicale U 186, Lille (France)
- OSTI ID:
- 7195296
- Journal Information:
- J. Virol.; (United States), Vol. 62:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FIBROBLASTS
BIOLOGICAL RADIATION EFFECTS
ONCOGENIC TRANSFORMATIONS
SENSITIVITY
NITRO COMPOUNDS
TOXICITY
SIMIAN VIRUS
CELL KILLING
COBALT 60
DNA REPLICATION
GAMMA RADIATION
GENE AMPLIFICATION
MAN
VIRUSES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CELL TRANSFORMATIONS
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEIC ACID REPLICATION
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PRIMATES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
SOMATIC CELLS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
560300 - Chemicals Metabolism & Toxicology