A relationship between proteinuria and acute tubulointerstitial disease in rats with experimental nephrotic syndrome

Journal Article · Wed May 01 00:00:00 EDT 1991 · American Journal of Pathology; (USA)

OSTI ID:5545691

Eddy, A A; McCulloch, L; Liu, E; Adams, J ^[1]

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1. Hospital for Sick Children, Toronto, ON (Canada)

The relationship between tubulointerstitial nephritis and proteinuria was characterized in experimental nephrosis in rats. In one group, proteinuria induced by aminonucleoside of puromycin (PAN) was reduced by using an 8% protein diet and adding the angiotensin I-converting enzyme (ACE) inhibitor enalapril to the drinking water. Two control groups were injected with saline and PAN, respectively, and fed a 27% protein diet. The first group had significantly reduced albuminuria and a definite attenuation of tubular cell injury. There was a strong positive correlation between the number of interstitial macrophages and albuminuria. The beneficial effect was reproduced by dietary-protein restriction alone, whereas ACE inhibition alone had an insignificant effect on the degree of proteinuria. Depletion of circulating T lymphocytes in one group of nephrotic rats eliminated interstitial lymphocytes but did not affect interstitial macrophage influx. Inhibition of the in situ proliferation of resident interstitial macrophages by unilateral kidney irradiation failed to change the intensity of the macrophage infiltration. Treatment of rats with sodium maleate produced proximal tubular cell toxicity but interstitial inflammation did not develop, suggesting that the latter is not a nonspecific response to tubular injury. These studies demonstrate a strong relationship between tubulointerstitial nephritis and the severity of proteinuria in experimental nephrosis.

• OSTI ID: 5545691
• Journal Information: American Journal of Pathology; (USA), Vol. 138:5; ISSN 0002-9440
• Country of Publication: United States
• Language: English

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