Metabolism of carcinogenic 2-hydroxybenzo(a)pyrene in rodent and human cells
- Tenn. Univ., Oak Ridge
The rates of metabolism of the carcinogenic 2-hydroxybenzo(a)pyrene (2-OH-B(a)P) and the noncarcinogenic 3- and 9-hydroxybenzo(a)pyrenes in cultured cell systems have been studied and compared. While 70 to 80% of the non-carcinogens are converted to water-soluble derivatives by hamster embryo fibroblasts in 24 h, carcinogenic 2-OH-B(a)P is metabolized at a slower rate (45% in 24 h), comparable to that for the parent hydrocarbon, benzo(a)pyrene (B(a)P). Analysis of extracellular organic solvent-soluble metabolites of 2-OH-B(a)P in cultured hamster embryo fibroblasts, using h.p.l.c., indicates the presence of a single major metabolite, which has been identified by mass spectroscopy as a dihydroxy derivative of B(a)P. At least one additional major organic solvent-soluble metabolite is formed in cultures of either mouse epidermal epithelial cells or human foreskin fibroblasts, indicating a different balance of metabolic pathways in these cell systems. The greater persistence of carcinogenic 2-OH-B(a)P in cells and its higher concentration in the cell cytoplasm compared with the noncarcinogenic phenols may be related to its relatively high biological activity. Differences in metabolism of 2-OH-B(a)P in several cultured cell systems indicate the importance of an appropriate choice of activating system in understanding the relationship between metabolism and carcinogenesis.
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5543549
- Journal Information:
- Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 1:12; ISSN CRNGD
- Country of Publication:
- United States
- Language:
- English
Similar Records
Metabolism and macromolecular binding of the carcinogen benzo(a)pyrene and its relatively inert isomer benzo(e)pyrene by hamster embryo cells
Benzo(a)pyrene carcinogenesis: a biochemical selection mechanism
Related Subjects
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
CARCINOGENESIS
CELL CULTURES
COMPARATIVE EVALUATIONS
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
EMBRYONIC CELLS
FIBROBLASTS
HAMSTERS
HYDROCARBONS
MAMMALS
METABOLISM
METABOLITES
ORGANIC COMPOUNDS
PATHOGENESIS
RODENTS
SOMATIC CELLS
VERTEBRATES