Efficient natural defense mechanisms against Listeria monocytogenes in T and B cell-deficient allogeneic bone marrow radiation chimeras. Preactivated macrophages are the main effector cells in an early phase after bone marrow transfer

Journal Article · Fri Sep 01 04:00:00 EDT 1989 · Journal of Immunology; (USA)

OSTI ID:5543150

Roesler, J; Groettrup, E B; Baccarini, M; Lohmann-Mattes, M L ^[1]

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1. Fraunhofer-Institut ITA, Hannover (Germany, F.R.)

Radiation chimeras in the early phase after bone marrow transplantation are a good model to study the efficiency of the body's nonspecific defense system represented by macrophages (M phi), polymorphonuclear cells (PMN), and NK cells. These cell types are present in large numbers in spleen and liver at that time, whereas the specific immune system represented by T and B cells is functionally deficient. We previously reported enhanced activities in vitro of M phi (and PMN) from recipient animals in an early phase after allogeneic bone marrow transfer. We here demonstrate that these activities result in enhanced spontaneous resistance against Listeria monocytogenes in vivo: CFU of L. monocytogenes in spleen and liver 48 h after infection were about 1 or 2 to 4 log steps less than in untreated control mice of donor or host haplotype. This enhanced resistance decreased over the 4-mo period after marrow transfer. Preactivated M phi were identified as the most important effector cells. Isolated from spleen and peritoneal cavity, they performed enhanced killing of phagocytosed Listeria. Such preactivated M phi occurred in recipient animals after transfer of allogeneic but not of syngeneic bone marrow. The precise mechanism of M phi activation in the allogeneic radiation chimera in the complete absence of any detectable T cell function is not clear at present. However, these preactivated M phi display an important protective effect against L. monocytogenes: chimeras could eliminate Listeria without acquisition of positive delayed-type sensitivity when infected with 10(3) bacteria. An inoculum of 5 . 10(3) L. monocytogenes resulted either in prolonged survival compared with normal mice of the recipient haplotype or in definitive survival accompanied by a positive delayed-type sensitivity.

• OSTI ID: 5543150
• Journal Information: Journal of Immunology; (USA), Journal Name: Journal of Immunology; (USA) Vol. 143:5; ISSN JOIMA; ISSN 0022-1767
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BACTERIA
BACTERIAL DISEASES
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW CELLS
CELL KILLING
CHIMERAS
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
DISEASE RESISTANCE
DISEASES
FUNCTIONS
GLANDS
GROWTH FACTORS
HEMIC DISEASES
IMMUNE SYSTEM DISEASES
IMMUNITY
INFECTIOUS DISEASES
LEUKOCYTES
LEUKOPENIA
LIVER
LYMPHOCYTES
LYMPHOKINES
LYMPHOPENIA
MACROPHAGES
MAMMALS
MATERIALS
MICE
MICROORGANISMS
MITOGENS
MOSAICISM
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTES
PROTEINS
RADIATION CHIMERAS
RODENTS
SOMATIC CELLS
SPLEEN
STEM CELLS
SURVIVAL TIME
TRANSPLANTS
VERTEBRATES