Macrophage function in murine allogeneic bone marrow radiation chimeras in the early phase after transplantation

Journal Article · Tue Aug 01 04:00:00 EDT 1989 · Journal of Leukocyte Biology; (USA)

OSTI ID:5513250

Roesler, J; Baccarini, M; Vogt, B; Lohmann-Matthes, M L ^[1]

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1. Fraunhofer-Institute for Toxicology and Aerosol Research, Hannover (Germany, F.R.)

We tested several of the functions of macrophages (M phi) in the early phase after allogeneic bone marrow transfer to get information about this important aspect of the nonspecific immune system in the T-cell-deficient recipient. On days 3-5 after transfer, the number of M phi was reduced in the spleen, liver, lungs, and peritoneal cavity (Pe). The phagocytosis of sheep red blood cells (SRBC) by these M phi was normal or even enhanced, as in the case of Pe-M phi. Already on days 8-12 after transfer, the number of M phi in spleen and liver exceeded that of controls, whereas the number was still reduced in lungs and Pe. We examined their ability to kill P815 tumor cells, to produce tumor necrosis factor-alpha (TNF alpha), to phagocytose SRBC, to produce reactive oxygen intermediates (ROI) in vitro and to kill Listeria monocytogenes in vivo. Most functions were normal and often even enhanced, depending on the organ origin, but the ability of Pe-M phi to produce ROI was reduced. Proliferative response to macrophage colony-stimulating factor (M-CSF) and killing of YAC-1 tumor cells revealed a high frequency of macrophage precursor cells in the spleen and liver and a high natural killer (NK) activity in the liver. Altogether, enhanced nonspecific immune function, especially preactivated M phi, may enable chimeras to survive attacks by opportunistic pathogens.

• OSTI ID: 5513250
• Journal Information: Journal of Leukocyte Biology; (USA), Journal Name: Journal of Leukocyte Biology; (USA) Vol. 46:2; ISSN JLBIE; ISSN 0741-5400
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BACTERIA
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW CELLS
CELL DIVISION
CELL KILLING
CHIMERAS
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
ELEMENTS
ERYTHROCYTES
FUNCTIONS
GLANDS
LEUKOCYTES
LIVER
LUNGS
LYMPHOCYTES
MACROPHAGES
MAMMALS
MATERIALS
MICE
MICROORGANISMS
MOSAICISM
NONMETALS
ORGANS
OXYGEN
PHAGOCYTES
PHAGOCYTOSIS
RADIATION CHIMERAS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
SPLEEN
TRANSPLANTS
TUMOR CELLS
VERTEBRATES