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Stimulation of T-cell activation by UV-treated, antigen-pulsed macrophages: evidence for a requirement for antigen processing and interleukin 1 secretion

Journal Article · · Cell. Immunol.; (United States)
;

The nature of the defect(s) in the ability of UV-treated guinea pig macrophages to stimulate the proliferative response of guinea pig T cells to soluble protein antigens was investigated. T cells proliferated vigorously when cultured with peritoneal exudate cells (PEC) which had been pulsed with soluble protein antigens, but failed to proliferate when cultured with soluble antigen or with antigen-pulsed, UV-treated PEC. UV-treated macrophages were unable to secrete interleukin 1 (IL-1). Addition of IL-1 partially restored the T-cell proliferative response stimulated by antigen-pulsed, UV-treated PEC. However, IL-1 was able to restore such a response only when the PEC were pulsed with antigen before being exposed to UV. Similar results were obtained when antigen-pulsed PEC were used to stimulate T cells to secrete interleukin 2 (IL-2). These results demonstrate that UV-treated macrophages are defective both in their ability to properly process and present antigen for T-cell recognition and in their ability to secrete IL-1.

Research Organization:
Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland
OSTI ID:
5533566
Journal Information:
Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 80:1; ISSN CLIMB
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
GUINEA PIGS
IMMUNE REACTIONS
LEUKOCYTES
LYMPHOCYTES
MACROPHAGES
MAMMALS
MATERIALS
MITOGENS
PHAGOCYTES
PHOTOSENSITIVITY
RADIATION EFFECTS
RADIATIONS
RODENTS
SENSITIVITY
SOMATIC CELLS
STIMULATION
ULTRAVIOLET RADIATION
VERTEBRATES