Preparation of radioactive acetyl-l-carnitine by an enzymatic exchange reaction
Journal Article
·
· Anal. Biochem.; (United States)
A rapid method for the preparation of (1-/sup 14/C)acetyl-L-carnitine is described. The method involves exchange of (1-/sup 14/C)acetic acid into a pool of unlabeled acetyl-L-carnitine using the enzymes acetyl-CoA synthetase and carnitine acetyltransferase. After isotopic equilibrium is attained, radioactive acetylcarnitine is separated from the other reaction components by chromatography on Dowex 1 (C1/sup -/) anion exchange resin. One of the procedures used to verify the product (1-/sup 14/C)acetyl-L-carnitine can be used to synthesize (3S)-(5-/sup 14/C)citric acid.
- Research Organization:
- Michigan State Univ., East Lansing
- OSTI ID:
- 5532422
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 119:2; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702* -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
ACETYL RADICALS
ACYL RADICALS
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBON 14
CARBON ISOTOPES
CARBOXYLIC ACIDS
CARNITINE
COENZYMES
ENZYMES
EVEN-EVEN NUCLEI
HYDROXY ACIDS
ISOTOPES
LABELLING
LIGHT NUCLEI
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADICALS
RADIOISOTOPES
SYNTHESIS
TRANSFERASES
USES
VITAMIN B GROUP
VITAMINS
YEARS LIVING RADIOISOTOPES
400702* -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
ACETYL RADICALS
ACYL RADICALS
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBON 14
CARBON ISOTOPES
CARBOXYLIC ACIDS
CARNITINE
COENZYMES
ENZYMES
EVEN-EVEN NUCLEI
HYDROXY ACIDS
ISOTOPES
LABELLING
LIGHT NUCLEI
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADICALS
RADIOISOTOPES
SYNTHESIS
TRANSFERASES
USES
VITAMIN B GROUP
VITAMINS
YEARS LIVING RADIOISOTOPES