Effect of (L-Carnitine) on acetyl-L-carnitine production by heart mitochondria
The authors recently reported a large efflux of acetyl-L-carnitine from rat heart mitochondria during state 3 respiration with pyruvate as substrate both in the presence and absence of malate. In this series of experiments, the effect of the concentration of L-carnitine on the efflux of acetyl-L-carnitine and on the production of /sup 14/CO/sub 2/ from 2-/sup 14/C-pyruvate was determined. Maximum acetylcarnitine production (approximately 25 n moles/min/mg protein) was obtained at 3-5 mM L-carnitine in the absence of added malate. /sup 14/CO/sub 2/ production decreased as the concentration of L-carnitine increased; it plateaued at 3-5 mM L-carnitine. These data indicate carnitine can stimulate flux of pyruvate through pyruvate dehydrogenase and can reduce flux of acetyl CoA through the Krebs cycle by acting as an acceptor of the acetyl moieties of acetyl CoA generated by pyruvate dehydrogenase.
- Research Organization:
- Michigan State Univ., East Lansing
- OSTI ID:
- 5097151
- Report Number(s):
- CONF-8606151-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CARNITINE
CELL CONSTITUENTS
CHALCOGENIDES
COENZYMES
DOSE-RESPONSE RELATIONSHIPS
ENZYMES
HEART
HYDROXY ACIDS
ISOTOPE APPLICATIONS
KETO ACIDS
KINETICS
LABELLED COMPOUNDS
MITOCHONDRIA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PYRUVIC ACID
REACTION KINETICS
RESPIRATION
SYNTHESIS
TRACER TECHNIQUES
VITAMIN B GROUP
VITAMINS