Synthesis of type IV procollagen in lung explants
Journal Article
·
· Am. Rev. Respir. Dis.; (United States)
OSTI ID:5531973
Pulmonary basement membranes are believed to play important roles in lung morphogenesis, in maintenance of lung alveolar architecture, and in repair after pulmonary injury. However, very little is known about the synthesis and matrix deposition of lung basement membrane macromolecules. Accordingly, we have investigated the synthesis of type IV procollagen, the major collagenous component of basement membranes, in slices of adult rat lung. After a 4-h labeling with (/sup 3/H)proline, type IV procollagen chains were extracted from lung homogenates with 2 M guanidine-HCl and purified by salt fractionation and ion exchange chromatography. The chains comigrated with authentic type IV chains by SDS-PAGE and were selectively coprecipitated with antibodies to type IV collagen. Peptide mapping confirmed their identity as pro alpha 1(IV) and pro alpha 2(IV), and suggested that the chains are predominantly assembled as heteropolymers. There was no evidence for proteolytic processing of the newly synthesized type IV procollagen even though type I and III procollagens were rapidly processed to lower molecular weight intermediates and collagen. Type IV procollagen accounted for approximately 40% of the extracted radiolabeled collagen, suggesting that there may be a relatively high turnover of lung basement membrane collagen in vivo.
- Research Organization:
- Washington Univ. Medical Center, St. Louis, MO
- OSTI ID:
- 5531973
- Journal Information:
- Am. Rev. Respir. Dis.; (United States), Journal Name: Am. Rev. Respir. Dis.; (United States) Vol. 4; ISSN ARDSB
- Country of Publication:
- United States
- Language:
- English
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Sun Sep 01 00:00:00 EDT 1991
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
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OSTI ID:5823122
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OSTI ID:6771100
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ANIMALS
ANTIBODIES
AZOLES
BIOSYNTHESIS
BODY
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHROMATOGRAPHY
COLLAGEN
GUANIDINES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HOMOGENATES
ION EXCHANGE CHROMATOGRAPHY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LUNGS
MAMMALS
MEMBRANES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RATS
RESPIRATORY SYSTEM
RODENTS
SCLEROPROTEINS
SEPARATION PROCESSES
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ANIMALS
ANTIBODIES
AZOLES
BIOSYNTHESIS
BODY
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHROMATOGRAPHY
COLLAGEN
GUANIDINES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HOMOGENATES
ION EXCHANGE CHROMATOGRAPHY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LUNGS
MAMMALS
MEMBRANES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RATS
RESPIRATORY SYSTEM
RODENTS
SCLEROPROTEINS
SEPARATION PROCESSES
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES