Rapid electronic autofluorography of labeled macromolecules on two-dimensional gels

Journal Article · Fri Mar 12 04:00:00 EST 1982 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.7063849· OSTI ID:5530346

Davidson, J B; Case, A L

The feasibility of electronically locating and measuring tritium-labeled macromolecules directly on dried electrophoretic gels has been demonstrated. This new procedure eliminates the usual long film exposure in autofluorography and the attendant delay in processing and data reduction. An image intensifier and electronic camera tube are used to integrate the light produced by the tritium interaction with a scintillator incorporated in the gel. Preliminary results show that, compared to film, the exposure is reduced 100 to 1000 times. The response to low activity levels is improved, and spatial resolution is maintained. A proposed instrument could be used for measuring other isotopes as well as fluorescent and visible stains. 2 figures.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5530346
• Journal Information: Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 215; ISSN SCIEA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOMEDICAL RADIOGRAPHY
CHEMICAL ANALYSIS
COLLOIDS
DIAGNOSTIC TECHNIQUES
DISPERSIONS
ELECTRON TUBES
ELECTROPHORESIS
FLUOROSCOPY
GELS
HYDROGEN ISOTOPES
IMAGE INTENSIFIERS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MATERIALS
MEDICINE
NUCLEAR MEDICINE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
RADIOISOTOPES
RADIOLOGY
TRITIUM
YEARS LIVING RADIOISOTOPES