Application of /sup 31/P-NMR spectroscopy to the study of striated muscle metabolism

Journal Article · Fri Jan 01 00:00:00 EST 1982 · Am. J. Physiol.; (United States)

OSTI ID:5530091

Meyer, R A; Kushmerick, M J; Brown, T R

This review presents the principles and limitations of phosphorus nuclear magnetic resonance (/sup 31/P-NMR) spectroscopy as applied to the study of striated muscle metabolism. Application of the techniques discussed include noninvasive measurement of high-energy phosphate, intracellular pH, intracellular free Mg/sup 2 +/, and metabolite compartmentation. In perfused cat biceps (fast-twitch) muscles, but not in soleus (slow-twitch), NMR spectra indicate a substantially lower (1 mM) free inorganic phosphate level than when measured chemically (6 mM). In addition, saturation and inversion spin-transfer methods that enable direct measurement of the unidirectional fluxes through creatine kinase are described. In perfused cat biceps muscle, results suggest that this enzyme and its substrates are in simple chemical equilibrium.

• OSTI ID: 5530091
• Journal Information: Am. J. Physiol.; (United States), Vol. 242:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MUSCLES
METABOLISM
NUCLEAR MAGNETIC RESONANCE
REVIEWS
CATS
ENZYMES
MAGNESIUM
METABOLITES
NMR SPECTRA
PH VALUE
PHOSPHATES
PHOSPHORUS 31
PHYSIOLOGY
ALKALINE EARTH METALS
ANIMALS
DOCUMENT TYPES
ELEMENTS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
METALS
NUCLEI
ODD-EVEN NUCLEI
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
RESONANCE
SPECTRA
STABLE ISOTOPES
VERTEBRATES
550501* - Metabolism- Tracer Techniques
551001 - Physiological Systems- Tracer Techniques