Repair of gamma-ray-induced DNA base damage in xeroderma pigmentosum cells
The repair of DNA damage produced by /sup 137/Cs gamma irradiation was measured with a preparation from Micrococcus luteus containing DNA damage-specific endonucleases in combination with alkaline elution. The frequency of these endonuclease sensitive sites (ESS) was determined after 54 or 110 Gy of oxic irradiation in normal and xeroderma pigmentosum (XP) fibroblasts from complementation groups A, C, D, and G. Repair was rapid in all cell strains with greater than 50% repair after 1.5 h of repair incubation. At later repair times, 12-17 h, more ESS remained in XP than in normal cells. The frequency of excess ESS in XP cells was approximately 0.04 per 10(9) Da of DNA per Gy which was equivalent to 10% of the initial ESS produced. The removal of ESS was comparable in XP cells with normal radiosensitivity and XP3BR cells which have been reported to be moderately radiosensitive.
- Research Organization:
- National Cancer Institute, Bethesda, MD
- OSTI ID:
- 5528916
- Journal Information:
- Radiat. Res.; (United States), Vol. 1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FIBROBLASTS
GENETIC RADIATION EFFECTS
XP CELLS
DNA REPAIR
RADIOSENSITIVITY
CELL CULTURES
CESIUM 137
DNA
DOSE-RESPONSE RELATIONSHIPS
ENDONUCLEASES
GAMMA RADIATION
MICROCOCCUS
XERODERMA PIGMENTOSUM
ALKALI METAL ISOTOPES
ANIMAL CELLS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CESIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DISEASES
DNA-ASE
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
GENETIC EFFECTS
HYDROLASES
IONIZING RADIATIONS
ISOTOPES
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RECOVERY
REPAIR
SKIN DISEASES
SOMATIC CELLS
YEARS LIVING RADIOISOTOPES
560121* - Radiation Effects on Cells- External Source- (-1987)