Repair of 254 nm ultraviolet-induced (6-4) photoproducts: monoclonal antibody recognition and differential defects in xeroderma pigmentosum complementation groups A, D, and variant

Journal Article · Wed Nov 01 00:00:00 EST 1989 · Journal of Investigative Dermatology; (USA)

DOI:https://doi.org/10.1111/1523-1747.ep12319907· OSTI ID:5259269

Hiramoto, T; Matsunaga, T; Ichihashi, M; Nikaido, O; Fujiwara, Y; Mishima, Y ^[1]

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1. Kobe Univ. (Japan)

Repair kinetics of ultraviolet (UV) light-induced (6-4) photoproducts in xeroderma pigmentosum complementation group A, D, and variant cells were studied by the enzyme-linked immunosorbent assay (ELISA) using a specific monoclonal antibody raised against (6-4) photoproducts, together with unscheduled DNA synthesis (UDS) and loss of T4 endonuclease V-susceptible sites (ESS). Group AXP35KO cells completely failed to repair both ESS (cyclobutane pyrimidine dimers) and antibody-recognizing (6-4) photoproducts until tested 24 h after irradiation, and had 2% early-time UDS. Group DXP43KO cells showed about 10% removal of both (6-4) photoproducts and ESS in 24 h, despite showing a residually higher level of 40% early-time and cumulative UDS. Thus, the results substantiated the extreme UV hypersensitivity of XP group A and D cells. However, XP52KO variant cells exhibited the normal level of UDS and ESS loss, but a slightly reduced repair of antibody-recognizing (6-4) photoproducts at 6 and 12 h after irradiation, which may account for a small UV hypersensitivity of the XP variant cells.

• OSTI ID: 5259269
• Journal Information: Journal of Investigative Dermatology; (USA), Vol. 93:5; ISSN 0022-202X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
MONOCLONAL ANTIBODIES
BIOLOGICAL FUNCTIONS
XP CELLS
DNA REPAIR
DOSE-RESPONSE RELATIONSHIPS
ENDONUCLEASES
ENZYME IMMUNOASSAY
GENETIC RADIATION EFFECTS
IN VITRO
MAN
PYRIMIDINE DIMERS
ULTRAVIOLET RADIATION
XERODERMA PIGMENTOSUM
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DISEASES
DNA-ASE
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
FUNCTIONS
GENETIC EFFECTS
HYDROLASES
IMMUNOASSAY
MAMMALS
PHOSPHODIESTERASES
PRIMATES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SKIN DISEASES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture