Development of methods for the structural characterization of complex carbohydrates. Technical progress report, September 1985-July 1986

Technical Report · Tue Jul 01 04:00:00 EDT 1986

OSTI ID:5528895

Albersheim, P

The sequence of the glycosyl residues in an oligosaccharide is often the most desired and challenging characteristic to elucidate. The development of a generalized method for determining the sequence of the glycosyl residues of complex carbohydrates is reported. The glycosyl-sequencing method developed uses high-performance liquid chromatography to separate the mixture of peralkylated oligoglycosyl alditols generated from the complex carbohydrates under study. Generally, the complex carbohydrate is first per-O-methylated, then more-or-less-randomly fragmented, usually by partial acid hydrolysis, and the resulting mixture of partially-O-methylated oligosccharides is reduced to form partially O-methylated oligoglycosyl alditols. Those hydroxyls not O-methylated were involved in the interconnections between the fragment; these are then usually marked by O-ethylation to form a mixture of partially O-ethylated, partially O-methylated oligoglycosyl alditols. The structures of these fragments overlap within the structure of the complex carbohydrate being characterized. The structures of more than enough overlapping fragments are obtained to elucidate the structure of the carbohydrate.

• Research Organization: Georgia Univ., Athens (USA). Complex Carbohydrate Research Center
• DOE Contract Number: FG09-85ER13424
• OSTI ID: 5528895
• Report Number(s): DOE/ER/13424-1; ON: DE86014157
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
CARBOHYDRATES
CHEMICAL REACTIONS
CHROMATOGRAPHY
DERIVATIZATION
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTROSCOPY
MOLECULAR STRUCTURE
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
SACCHARIDES
SEPARATION PROCESSES
SPECTROSCOPY